Biodegradation of microcystins by bacterial communities co-existing with the flagellate Monas guttula and concurrent succession of community structures
Biodegradation of microcystins by bacterial communities co-existing with the flagellate Monas guttula and concurrent succession of community structures
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DOI：
10.2166/aqua.2011.069
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发表时间：
2011-01-01
影响因子：
4.3
通讯作者：
Sugiura, Norio
中科院分区：
文献类型：
--
作者：
Li, Jieming;Shimizu, Kazuya;Sugiura, Norio
点单胞藻对微囊藻的摄食可同时降解培养液中微囊藻产生的微囊藻毒素。虽然与M.虽然已经分离到了M. guttula，但在与M. guttula共存的土著细菌群落中，MC降解菌是否能降解MC仍是未知的。点滴为了研究这一点，我们从M.点滴培养，以测试每个社区降解MC的能力。结果表明，MCs迅速降解到检测不到的水平，和早期MC耗尽由于生物降解后，与MCs在两个社区重新扣球明显。这些结果表明，MC降解菌在两个群落中都有分布，并且可以降解与M共存的土著细菌群落中的MC。点滴变性梯度凝胶电泳（DGGE）揭示了物种多样性和结构之间的差异，两个社区。DGGE图谱聚类分析表明，细胞束缚群落结构对退化过程的响应比自由生活群落更敏感，两种群落结构在退化过程中沿着遗传进化关系更密切。
Grazing on Microcystis by the flagellate Monas guttula causes simultaneous degradation of microcystins (MCs) produced by Microcystis in culture. Although the MC-degrading bacterial strains that co-exist with M. guttula have been isolated, it is still unknown if the MC-degrading bacteria can degrade MCs within the indigenous bacterial community co-existing with M. guttula. To investigate this, we separated two indigenous bacterial communities (free-living and cell-bound) from M. guttula culture to test the ability of each community to degrade MCs. Results showed that MCs were rapidly degraded to undetectable level, and earlier MC exhaustion due to biodegradation was evident after re-spiking with MCs in both communities. These findings show that the MC-degrading bacteria are distributed over both communities, and can degrade MCs within the indigenous bacterial community co-existing with M. guttula. Denaturing gradient gel electrophoresis (DGGE) revealed differences in species diversity and structure between the two communities. Cluster analysis for DGGE patterns indicated that cell-bound community structures responded more sensitively than free-living community during degradation, and the two community structures evolved closer genetically with each other along the degradation period.