Inactivation of Coronaviruses and Phage Phi6 from Irradiation across UVC Wavelengths
Inactivation of Coronaviruses and Phage Phi6 from Irradiation across UVC Wavelengths
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DOI：
10.1021/acs.estlett.1c00178
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发表时间：
2021-03-17
影响因子：
10.9
通讯作者：
Linden, Karl G.
中科院分区：
文献类型：
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作者：
Ma, Ben;Linden, Yarrow S.;Linden, Karl G.
发射UVC辐射（200-280 nm）的紫外线（UV）设备已被证明对病毒消毒有效，特别是在表面和空气中，因为它们的快速有效性和限制没有材料腐蚀。许多UV诱导灭活的研究集中在无包膜病毒上。关于UVC在UVC波长范围内对包膜病毒的作用知之甚少。在这项研究中，我们确定了两种冠状病毒（ssRNA）和包膜dsRNA噬菌体替代品在缓冲水溶液（pH 7.4）中的灭活效率，使用五种常用的UVC设备，独特地发射不同波长的光，从222 nm发射的氯化氪（KrCl*）准分子到282 nm发射的UVC LED。我们的研究结果表明，使用UVC设备可以有效地灭活包膜病毒，其中KrCl* 准分子具有最好的消毒性能（即，最高灭活率）。冠状病毒在整个UVC范围内对紫外线照射表现出相似的敏感性，而噬菌体替代物的抗性要高得多，对远UVC（
Ultraviolet (UV) devices emitting UVC irradiation (200-280 nm) have proven to be effective for virus disinfection, especially on surfaces and in air, due to their rapid effectiveness and limited to no material corrosion. Numerous studies of UV-induced inactivation focused on nonenveloped viruses. Little is known about UVC action on enveloped viruses across UVC wavelengths. In this study, we determined inactivation efficiencies of two coronaviruses (ssRNA) and an enveloped dsRNA bacteriophage surrogate in buffered aqueous solution (pH 7.4) using five commonly available UVC devices that uniquely emit light at different wavelengths spanning 222 nm emitting krypton chloride (KrCl*) excimers to 282 nm emitting UVC LEDs. Our results show that enveloped viruses can be effectively inactivated using UVC devices, among which the KrCl* excimer had the best disinfection performance (i.e., highest inactivation rate) for all three enveloped viruses. The coronaviruses exhibited similar sensitivities to UV irradiation across the UVC range, whereas the bacteriophage surrogate was much more resistant and exhibited significantly higher sensitivity to the Far UVC (