Tobacco Translationally Controlled Tumor Protein Interacts with Ethylene Receptor Tobacco Histidine Kinase1 and Enhances Plant Growth through Promotion of Cell Proliferation1[OPEN]
Tobacco Translationally Controlled Tumor Protein Interacts with Ethylene Receptor Tobacco Histidine Kinase1 and Enhances Plant Growth through Promotion of Cell Proliferation1[OPEN]
复制标题
DOI：
10.1104/pp.15.00355
复制
发表时间：
2015-05
期刊：
影响因子：
7.4
通讯作者：
Jian-Jun Tao;Yang-Rong Cao;Hao-Wei Chen;Wei Wei-Wei;Qing-Tian Li;B. Ma;Wan-Ke Zhang;S. Chen
中科院分区：
文献类型：
--
作者：
Jian-Jun Tao;Yang-Rong Cao;Hao-Wei Chen;Wei Wei-Wei;Qing-Tian Li;B. Ma;Wan-Ke Zhang;S. Chen
翻译控制的肿瘤蛋白与内质网上的一类乙烯受体结合并影响蛋白质降解。乙烯是一种重要的植物激素，在整个生命周期中调节植物的生长、发育和胁迫反应。以前，我们发现，亚家族II乙烯受体烟草（烟草）组氨酸激酶1（NTHK 1）促进幼苗生长。在这里，我们确定了NTHK 1相互作用的蛋白质，通过酵母（酿酒酵母）的双杂交试验，并进一步表征其在植物生长中的作用，在功能上控制的肿瘤蛋白（NtTCTP）。通过体外谷胱甘肽S-转移酶下拉和体内免疫共沉淀和双分子荧光互补试验进一步证实了这种相互作用，NTHK 1的激酶结构域介导了与NtTCTP的相互作用。NtTCTP蛋白由乙烯处理诱导，并与NTHK 1共定位于内质网。NtTCTP或NTHK 1的过表达降低植物对乙烯的反应并促进幼苗生长，主要通过加速细胞增殖。遗传分析表明NtTCTP是NTHK 1功能所必需的。此外，NtTCTP的缔合防止NTHK 1从蛋白酶体介导的蛋白质降解。我们的数据表明，由乙烯引发的植物生长抑制是由一个独特的反馈机制，其中乙烯诱导的NtTCTP与乙烯受体NTHK 1的关联和稳定，以减少植物对乙烯的反应，并通过加速细胞增殖促进植物生长。
Translationally-controlled tumor protein binds with a class of ethylene receptors at the endoplasmic reticulum and affects protein degradation. Ethylene is an important phytohormone in the regulation of plant growth, development, and stress response throughout the lifecycle. Previously, we discovered that a subfamily II ethylene receptor tobacco (Nicotiana tabacum) Histidine Kinase1 (NTHK1) promotes seedling growth. Here, we identified an NTHK1-interacting protein translationally controlled tumor protein (NtTCTP) by the yeast (Saccharomyces cerevisiae) two-hybrid assay and further characterized its roles in plant growth. The interaction was further confirmed by in vitro glutathione S-transferase pull down and in vivo coimmunoprecipitation and bimolecular fluorescence complementation assays, and the kinase domain of NTHK1 mediates the interaction with NtTCTP. The NtTCTP protein is induced by ethylene treatment and colocalizes with NTHK1 at the endoplasmic reticulum. Overexpression of NtTCTP or NTHK1 reduces plant response to ethylene and promotes seedling growth, mainly through acceleration of cell proliferation. Genetic analysis suggests that NtTCTP is required for the function of NTHK1. Furthermore, association of NtTCTP prevents NTHK1 from proteasome-mediated protein degradation. Our data suggest that plant growth inhibition triggered by ethylene is regulated by a unique feedback mechanism, in which ethylene-induced NtTCTP associates with and stabilizes ethylene receptor NTHK1 to reduce plant response to ethylene and promote plant growth through acceleration of cell proliferation.