Relationships Between Uterine Blood Flow, Peripheral Sex Steroids, Expression of Endometrial Estrogen Receptors and Nitric Oxide Synthases During the Estrous Cycle in Mares
Relationships Between Uterine Blood Flow, Peripheral Sex Steroids, Expression of Endometrial Estrogen Receptors and Nitric Oxide Synthases During the Estrous Cycle in Mares
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DOI：
10.1262/jrd.10-023t
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发表时间：
2011-02-01
影响因子：
1.8
通讯作者：
Bollwein, Heinrich
中科院分区：
文献类型：
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作者：
Honnens, Aenne;Weisser, Simone;Bollwein, Heinrich
本研究旨在探讨5只马驹发情周期子宫灌注与雌激素、孕酮及子宫一氧化氮合酶（NOS）系统的关系。在一个发情周期的第0天（排卵）、第1天、第5天、第11天和第15天以及发情期间（第1天至第4天）每日进行彩色多普勒超声测量子宫血流和采血测定血浆雌激素和孕酮浓度；于第0、1、5、11、15和3天采集子宫内膜活检，分析NOS和雌激素受体mRNA表达。通过计算平均时间平均最大流速（TAMV）和脉搏指数（PI）评估子宫各动脉血流。采用特异性酶免疫法测定血浆雌激素和孕酮浓度。采用逆转录-聚合酶链式反应（RT-PCR）法定量检测内皮细胞NOS （eNOS）、诱导型NOS （iNOS）及雌激素受体α (ER α)和β (ER β) mRNA的表达。在发情周期中TAMV和PI呈双相模式(P
The objective of this study was to investigate the relationships between uterine perfusion and estrogen, progesterone and the uterine nitric oxide synthase (NOS) system in five trotter mares during the estrous cycle. Color Doppler sonography for measurement of uterine blood flow and collection of blood for determination of plasma estrogen and progesterone concentrations were performed on days 0 (= ovulation), 1, 5, 11 and 15 and daily during estrus (days -1 to -4) of one estrous cycle; endometrial biopsy collection for mRNA expression analysis of NOS and estrogen receptors was performed on days 0, 1, 5, 11, 15 and -3. Blood flow in each uterine artery was assessed by calculating the mean time-averaged maximum velocity (TAMV) and the pulsatility index (PI). Plasma concentrations of estrogen and progesterone were determined using specific enzyme immunoassays. The mRNA expressions of endothelial NOS (eNOS), inducible NOS (iNOS) as well as estrogen receptors alpha (ER alpha) and beta (ER beta) were quantified using reverse transcriptase-polymerase chain reaction (RT-PCR) analysis. The TAMV and PI had a biphasic pattern during the estrous cycle (P