Optical imaging of cancer-related proteases using near-infrared fluorescence matrix metalloproteinase-sensitive and cathepsin B-sensitive probes.
Optical imaging of cancer-related proteases using near-infrared fluorescence matrix metalloproteinase-sensitive and cathepsin B-sensitive probes.
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作者：
Yhee JY;Kim SA;Koo H;Son S;Ryu JH;Youn IC;Choi K;Kwon IC;Kim K
组织蛋白酶B和基质金属蛋白酶(MMPs)通过控制细胞外基质的降解，在肿瘤进展中起关键作用。因此，这些蛋白水解酶在癌症研究中受到了极大的关注，并开发了许多利用这些蛋白水解酶的成像探针。我们的团队开发了基于聚合物纳米颗粒平台的组织蛋白酶B和基质金属蛋白酶成像纳米探针。组织蛋白酶B和基质金属蛋白酶成像探针均使用近红外荧光(NIRF)染料和暗猝灭剂以获得高灵敏度，并且每个探针中的蛋白酶敏感的多肽序列使探针具有较高的特异性。我们比较了组织蛋白酶B和基质金属蛋白酶敏感探针在癌症相关环境中的生物活性，以研究这两种探针的生物学特性。结果表明，组织蛋白酶B探针进入细胞质后出现荧光恢复现象。这一特性可用于评价利用探针偶联纳米粒在体内的胞质靶向递送。另一方面，在体内和组织研究中，基质金属蛋白酶探针具有更好的特异性。这项比较研究将提供基于多肽的光学探针的准确信息，并允许它们适当地应用于癌症诊断。
Cathepsin B and matrix metalloproteinase (MMP) play key roles in tumor progression by controlled degradation of extracellular matrix. Consequently, these proteases have been attracted in cancer research, and many imaging probes utilizing these proteases have been developed. Our groups developed cathepsin B and MMP imaging nanoprobes based on polymer nanoparticle platform. Both cathepsin B and MMP imaging probes used near-infrared fluorescence (NIRF) dye and dark-quencher to for high sensitivity, and protease-sensitive peptide sequence in each probe authorized high specificity of the probes. We compared the bioactivities of cathepsin B and MMP sensitive probes in cancer-related environments to investigate the biological property of the probes. As a result, cathepsin B probe showed fluorescence recovery after the probe entered the cytoplasm. This property could be useful to evaluate the cytoplasmic targeted delivery by using probe-conjugated nanoparticles in vivo. On the other hand, MMP probe was superior in specificity in vivo and tissue study. This comparative study will provide precise information about peptide-based optical probes, and allow their proper application to cancer diagnosis.
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影响因子：
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作者：
Ryu, Ju Hee;Kim, Sun Ah;Kim, Kwangmeyung
通讯作者：
Kim, Kwangmeyung
影响因子：
14.8
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Blum, Galia;von Degenfeld, Georges;Bogyo, Matthew
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Bogyo, Matthew
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64.5
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Brooks, PC;Stromblad, S;Cheresh, DA
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Cheresh, DA
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10.8
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Lee S;Ryu JH;Park K;Lee A;Lee SY;Youn IC;Ahn CH;Yoon SM;Myung SJ;Moon DH;Chen X;Choi K;Kwon IC;Kim K
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Kim K
影响因子：
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Kim GB;Kim YP
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Kim YP