Generation and characterization of a human induced pluripotent stem cell (iPSC) line from a patient with congenital heart disease (CHD).
Generation and characterization of a human induced pluripotent stem cell (iPSC) line from a patient with congenital heart disease (CHD).
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DOI：
10.1016/j.scr.2022.102958
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发表时间：
2022-12
影响因子：
1.2
通讯作者：
McBride, Kim L.
中科院分区：
文献类型：
--
作者：
Lin, Hui;Ye, Shi-Qiao;Xu, Zhao-Hui;Penaloza, Jacqueline S.;Aljuhani, Mona;Vetter, Tatyana;Zhao, Ming -Tao;McBride, Kim L.
Epstein-Barr 病毒 (EBV) 永生化淋巴母细胞系 (LCL) 广泛用于银行库。这种生物资源可用于创建人类 iPSC 系来模拟包括先心病在内的疾病。我们从先天性主动脉瓣狭窄患者身上生成了 LCL 衍生的 iPSC 系 (NCHi001-A)。 NCHi001-A不含EBV和转基因，表现出干细胞样形态，表达多能性标记，具有正常核型，并且可以在体外分化为三个胚层细胞。通过基于微阵列的分析进行的关系推断表明 NCHi001-A 与亲本细胞系相同。 NCHi001-A 可用于疾病建模、药物发现和细胞疗法开发。
Epstein-Barr virus (EBV) immortalized lymphoblastoid cell lines (LCLs) are widely used for banking. This bioresource could be leveraged for creating human iPSC lines to model diseases including CHD. We generated an LCL-derived iPSC line (NCHi001-A) from a patient with congenital aortic valve stenosis. NCHi001-A was EBV and transgenes free, exhibited stem cell-like morphology, expressed pluripotency markers, has a normal karyotype, and could be differentiated into cells of three germ layers in vitro. Relationship inference via a microarray-based analysis showed NCHi001-A is identical to the parental cell line. NCHi001-A can be used for disease modeling, drug discovery, and cell therapy development.
影响因子：
1.2
作者：
Martineau, Laurie;Racine, Veronique;Puymirat, Jack
通讯作者：
Puymirat, Jack
影响因子：
5.5
作者：
Lin H;McBride KL;Garg V;Zhao MT
通讯作者：
Zhao MT
DOI：
10.1073/pnas.1708991114
发表时间：
2017-12-26
影响因子：
11.1
作者：
Zhao, Ming-Tao;Chen, Haodong;Wu, Joseph C.
通讯作者：
Wu, Joseph C.