Lactobacillus acidophilus S-layer protein-mediated inhibition of PEDV-induced apoptosis of Vero cells
Lactobacillus acidophilus S-layer protein-mediated inhibition of PEDV-induced apoptosis of Vero cells
复制标题
嗜酸乳杆菌 S 层蛋白介导的 PEDV 诱导的 Vero 细胞凋亡抑制
DOI：
10.1016/j.vetmic.2019.01.003
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发表时间：
2019-02-01
影响因子：
3.3
通讯作者：
Hou, Jibo
中科院分区：
文献类型：
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作者：
Zhang, Xiaoyan;Li, Pengcheng;Hou, Jibo
为深入了解嗜酸乳杆菌的作用机理。为了研究猪流行性腹泻病毒（PEDV）S-层蛋白的抗病毒活性，我们研究了S-层蛋白如何影响PEDV感染和PEDV诱导的Vero细胞凋亡。用S层蛋白对PEDV感染的Vero细胞进行预处理（排除测定）、共孵育（竞争测定）和后处理（置换测定）。有趣的是，仅在排除测定中观察到S层蛋白对PEDV的显著抑制。在PEDV感染的Vero细胞中，我们发现细胞凋亡是由caspase-8和caspase-3在感染后期激活介导的。当PEDV感染的Vero细胞与S-层蛋白的预处理，Vero细胞凋亡率显着下降，细胞损伤显着减少，通过流式细胞术和显微镜评价。详细的分析表明，S层蛋白抑制caspase-8和caspase-3的活性。综上所述，我们的结果表明，L。嗜酸乳杆菌S层蛋白在PEDV感染Vero细胞过程中起抑制作用，并且该蛋白的拮抗活性不是通过与PEDV竞争结合位点。此外，研究结果表明，L。嗜酸性S层蛋白通过减少感染后期的半胱天冬酶-8和半胱天冬酶-3激活来防止PEDV诱导的细胞凋亡。这一机制可能代表了一种新的方法来拮抗PEDV和其他病毒。
To gain insight into the mechanism of Lactobacillus acidophilus (L. acidophilus) S-layer protein antiviral activity, we examined how S-layer protein impacts porcine epidemic diarrhea virus (PEDV) infection and PEDV-induced apoptosis of Vero cells. Pretreatment (exclusion assay), coincubation (competition assay), and post-treatment (displacement assay) of PEDV-infected Vero cells with the S-layer protein was examined. Interestingly, significant inhibition of PEDV by S-layer protein was only observed in the exclusion assay. In Vero cells infected with PEDV, we found that apoptosis was mediated by activation of caspase-8 and caspase-3 in the late stage of infection. When PEDV-infected Vero cells were pretreated with S-layer protein, rates of Vero cell apoptosis were markedly decreased and cell damage was significantly reduced, as evaluated by flow cytometry and microscopy. Detailed analyses showed that the S-layer protein inhibited caspase-8 and caspase-3 activity. Taken together, our results suggest that L. acidophilus S-layer protein plays an inhibitory role during PEDV infection of Vero cells, and that the antagonistic activity of the protein is not via competition with PEDV for binding sites. In addition, the findings suggest that L. acidophilus S-layer protein protects against PEDV-induced apoptosis through reduced caspase-8 and caspase-3 activation in the later stages of infection. This mechanism may represent a novel approach for antagonizing PEDV and other viruses.