A new Caryospora coccidian species (Apicomplexa: Eimeriidae) from the laughing kookaburra (Dacelo novaeguineae)
A new Caryospora coccidian species (Apicomplexa: Eimeriidae) from the laughing kookaburra (Dacelo novaeguineae)
复制标题
DOI：
10.1016/j.exppara.2014.07.008
复制
发表时间：
2014-10-01
影响因子：
2.1
通讯作者：
Ryan, Una
中科院分区：
文献类型：
--
作者：
Yang, Rongchang;Brice, Belinda;Ryan, Una
本文报道了从笑翠鸟（Dacelo novaeguineae）中发现的一个核孢子球虫新种。孢子形成的卵囊（n = 30）呈卵形，卵囊壁光滑、无色、双层，大小为31.4 × 29.3（30.0-32.0 × 28.0-31.0）μ m，形状指数为1.1。卵囊含有一个球形至亚球形孢子囊，21.2 x 20.6（20.0-24.0 x 20.0-21.0）μ m。孢子囊残留物呈球形，无珠孔、Stieda、substieda和parastieda小体。蠕虫状子孢子（n = 8）平行或随机排列在孢子囊中，尺寸为17.0 × 4.8（16.0-18.0 × 4.0-6.0）μ m，L/W比为3.5。在子孢子的中间有一个大的球形的、后部的触肢体。在形态学上，该新种与核孢菌属（Caryospora）最为相似。上午和中午后采样的鸟类中，这种寄生虫的患病率分别为6.7%和33.3%。在两个位点进行进一步的分子表征; 18 S和28 S核糖体RNA（rRNA）。在18 S位点上，新种与Besnoitia besnoiti（99.2%）和Hammondia triffittae（98.8%）的亲缘关系最近。虽然在GenBank中没有获得核孢霉属的28 S部分序列，但与B的同源性最高。besnoiti（91.3%）。根据形态学和分子数据，这种球虫寄生虫是一个新的物种，迄今尚未报道。新的球虫寄生虫被命名为Caryospora daceloe n. sp.在其寄主D.笑翠鸟（laughing kookaburra）皇冠版权所有（C）2014由Elsevier Inc.发布。All rights reserved.
A new Caryospora coccidian species is described from the laughing kookaburra (Dacelo novaeguineae). Sporulated oocysts (n = 30) are ovoid in shape with a smooth, colourless, bilayered oocyst wall and measure 31.4 x 29.3 (30.0-32.0 x 28.0-31.0) mu m with a shape index of 1.1. Oocysts contain one spheroidal to subspheroidal sporocyst, 21.2 x 20.6 (20.0-24.0 x 20.0-21.0) mu m. A spheroidal shaped sporocyst residuum is present; micropyle, Stieda, substieda and parastieda bodies are absent. Vermiform sporozoites (n = 8) are arranged either parallel or randomly in the sporocyst, measuring 17.0 x 4.8 (16.0-18.0 x 4.0-6.0) mu m, with a L/W ratio of 3.5. There is a large spheroidal, posterior refractile body in the middle of the sporozoite. Morphologically, this new species is most similar to Caryospora. The prevalence of this parasite was 6.7% in birds sampled in the morning and 33.3% from those sampled after midday. Further molecular characterisation was conducted at two loci; the 18S and 28S ribosomal RNA (rRNA). At the 18S locus, the new species of Caryospora was most closely related to Besnoitia besnoiti (99.2% similarity) and Hammondia triffittae (98.8% similarity). Although, no 28S partial sequences from Caryospora were available in GenBank, the highest similarity was with B. besnoiti (91.3%). Based on morphological and molecular data, this coccidian parasite is a new species that to date has not been reported. The new coccidian parasite is named Caryospora daceloe n. sp. after its host D. novaeguineae (the laughing kookaburra). Crown Copyright (C) 2014 Published by Elsevier Inc. All rights reserved.