Bacterial diversity of cosmopolitan Culex pipiens and invasive Aedes japonicus from Germany
Bacterial diversity of cosmopolitan Culex pipiens and invasive Aedes japonicus from Germany
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DOI：
10.1007/s00436-017-5466-2
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发表时间：
2017-05
影响因子：
2
通讯作者：
Sina Zotzmann;Antje Steinbrink;K. Schleich;F. Frantzmann;Chinhda Xoumpholphakdy;Manuela Spaeth;C. V. Moro-C.
中科院分区：
文献类型：
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作者：
Sina Zotzmann;Antje Steinbrink;K. Schleich;F. Frantzmann;Chinhda Xoumpholphakdy;Manuela Spaeth;C. V. Moro-C.
近年来，共生细菌得到了极大的关注。例如，某些蚊子物种的微生物群似乎影响病原体的发展和传播。此外，已经进行了几次尝试，使用细菌作为副转基因工具，以帮助控制蚊媒疾病。本研究对野外捕获的库蚊成虫（Culex（Cx.）pipiens和实验室饲养的成蚊Aedes aerobic（Ae.在德国，使用非培养方法。从每个样本中提取基因组DNA，并进行真细菌16 S rDNA的PCR扩增。克隆反应后，每个样品选择28个含有16 S rDNA插入片段的细菌转化体/样品用于测序。对Cx. pipiensas以及Ae. japonicus的细菌群落多样，包括一些常见的细菌属。Blast分析鉴定了23例Cx标本中的21个细菌属，分属于2门。pipiens。第14章分析japonicus 3门11属。在这两种蚊子中，鉴定的分离株主要是变形菌门。在两种蚊子中只发现了4种细菌属，其中最流行的是Sphingomonas和RahnellainCx。pipiens和inAe. japonicus分别为。在我们的研究中发现的大多数细菌属以前已经在其他蚊子物种中被鉴定出来。由于目前缺乏数据，迫切需要对非常丰富的细菌属或种进行持续的检查，以确定它们与蚊子的生物学和适应性的相关性，包括病原体-宿主关系。
Symbiotic bacteria have gained significant attention in recent years. For example, microbiota of some mosquito species seems to influence the development and transmission of pathogens. Furthermore, several attempts using bacteria as a paratransgenetic tool have been made in order to assist the control of mosquito-borne diseases. In this study, we examined the bacterial diversity of wild-caught adultCulex(Cx.)pipiensand laboratory-reared adultAedes japonicus(Ae. japonicus) in Germany using a culture-independent method. Genomic DNA was extracted from each specimen and submitted to PCR amplification of eubacterial 16S rDNA. After the cloning reaction, 28 bacterial transformants per sample containing the 16S rDNA inserts were selected per each sample for sequencing. The analysed specimens ofCx. pipiensas well as ofAe. japonicusshowed a diverse bacterial community including some common bacterial genera. Blast analysis allowed to identify 21 bacterial genera belonging to 2 phyla among the 23 specimens ofCx. pipiens. The 14 analysedAe. japonicusrevealed 11 bacterial genera belonging to 3 phyla. In both mosquito species, identified isolates were mainly Proteobacteria. Only 4 of the bacterial genera were found in both mosquito species, with the most prevalent generaSphingomonasandRahnellainCx. pipiensand inAe. japonicusrespectively. Most of the bacterial genera found in our study have been identified in other mosquito species before. Due to the currently scarce data situation, ongoing examinations on the very abundant bacterial genera or species are strongly required to determine their relevance for the biology and adaptiveness of mosquitoes including pathogen-host relationship.