In Vitro and In Vivo Blood-Brain Barrier Models to Study West Nile Virus Pathogenesis.
In Vitro and In Vivo Blood-Brain Barrier Models to Study West Nile Virus Pathogenesis.
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DOI：
10.1007/978-1-4939-3670-0_9
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Nerurkar VR
中科院分区：
文献类型：
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作者：
Kumar M;Nerurkar VR
血脑屏障（BBB）是外周血液循环和中枢神经系统之间的特殊界面，专门调节两者之间的分子和细胞通量。它对于维持脑部止血起着至关重要的作用。血脑屏障限制病原体进入大脑，因此其通透性是决定病原体中枢作用的关键因素。一旦血脑屏障的通透性受到损害，就会对包括西尼罗病毒（WNV）疾病在内的许多脑部疾病的病因学产生严重影响。在本章中，我们描述了用于研究 WNV 发病机制的单层和双层体外 BBB 模型的制备、维护、感染和渗透性测量的方案。我们还描述了伊文思蓝染料测定法，这是一种在 WNV 感染后测试体内血管通透性的成熟方法。
The blood–brain barrier (BBB), a specialized interface between the peripheral blood circulation and the central nervous system, specifically regulates molecular and cellular flux between the two. It plays a critical role in the maintenance of brain hemostasis. The BBB restricts the entry of pathogens into the brain, and thus its permeability is a critical factor that determines their central effects. Once the permeability of BBB is compromised, it has serious implications in the etiology of many brain pathologies including West Nile virus (WNV) disease. In this chapter, we describe protocols for preparation, maintenance, infection and permeability measurement of monolayer and bilayer in vitro BBB models to study WNV pathogenesis. We also describe Evans blue dye assay, a well-established method to test vascular permeability in vivo after WNV infection.