Antiviral activity of cuprous oxide nanoparticles against Hepatitis C Virus in vitro
Antiviral activity of cuprous oxide nanoparticles against Hepatitis C Virus in vitro
复制标题
氧化亚铜纳米颗粒对丙型肝炎病毒的体外抗病毒活性
DOI：
10.1016/j.jviromet.2015.06.010
复制
发表时间：
2015-09-15
影响因子：
3.1
通讯作者：
Xu, Wensheng
中科院分区：
文献类型：
--
作者：
Hang, Xiaofeng;Peng, Haoran;Xu, Wensheng
小分子抑制剂联合或不联合干扰素可改善针对丙型肝炎病毒（HCV）感染的持续抗病毒应答。尽管如此，由于病毒的高突变率，预计对这些抑制剂的耐药性将迅速出现。因此，迫切需要新的抗病毒药物与目前可用的疗法组合来治疗HCV感染。在本研究中，我们评估了氧化亚铜纳米颗粒（CO-NPs）在HCV-DNA/Huh7.5.1细胞培养系统中对HCV的抗病毒效力。CO-NP能够在非细胞毒性浓度下显著抑制HC 1的感染性。此外，CO-NP抑制HCV假颗粒（HCVpp）的进入，包括基因型1a，1b和2a，而对HCV复制没有影响。进一步的添加时间实验表明，CO-NPs在附着和进入阶段都阻断了HCV感染。总之，我们报道了CO-NPs可以通过靶向感染性HCV颗粒与肝细胞的结合和病毒进入细胞来作为抗HCV剂。这些研究结果表明，CO-NPs可能在治疗慢性丙型肝炎患者中发挥新的作用。(C)2015 Elsevier B. V.版权所有。
Small molecular inhibitors in combination with or without interferon have improved sustained antiviral responses against Hepatitis C Virus (HCV) infection. Nonetheless, resistance to these inhibitors is expected to emerge rapidly due to the high mutation rate of the virus. Thus, new antiviral drugs, in combination with currently available therapies, are urgently needed to treat HCV infection. In the present study, we evaluated the antiviral efficacy of cuprous oxide nanoparticles (CO-NPs) against HCV in the HCVcc/Huh7.5.1 cell culture system. CO-NPs were able to significantly inhibit the infectivity of HCVcc at a non-cytotoxic concentration. In addition, CO-NPs inhibited the entry of HCV pseudoparticle (HCVpp), including genotypes 1a, 1b, and 2a, while no effect on HCV replication was observed. Further time-of-addition experiment indicated that CO-NPs blocked HCV infection both at the attachment and entry stages. In conclusion, we report that CO-NPs can act as an anti-HCV agent by targeting the binding of infectious HCV particles to hepatic cells and the virus entry into the cells. These findings suggest that CO-NPs may have novel roles in the treatment of patients with chronic hepatitis C. (C) 2015 Elsevier B.V. All rights reserved.