Genetic and physicochemical analyses of a novel ferret hepatitis E virus, and clinical signs of infection after birth
Genetic and physicochemical analyses of a novel ferret hepatitis E virus, and clinical signs of infection after birth
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DOI：
10.1016/j.meegid.2017.03.026
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发表时间：
2017-07-01
影响因子：
3.2
通讯作者：
Wakita, Takaji
中科院分区：
文献类型：
--
作者：
Li, Tian-Cheng;Yoshizaki, Sayaka;Wakita, Takaji
从美国雪貂养殖场进口的 9 只实验室雪貂 (Mustela putorius faro) 中检测到了 5 种新的雪貂戊型肝炎病毒 (HEV) 毒株。我们对雪貂 HEV RNA 和抗 HEV 抗体的检测以及丙氨酸转氨酶 (ALT) 值评估表明，所有 9 只雪貂都感染了雪貂 HEV，并且感染表现出 3 种症状。 模式：亚临床感染（n = 2）、急性肝炎（n = 6）和持续感染（n = 1）。对这五个品系的全基因组序列进行的下一代序列分析显示，它们的核苷酸序列同一性在99.5%至99.9%之间，表明遗传相似的雪貂HEV曾在美国雪貂养殖场流通。相比之下，这些毒株与从荷兰 (JN998607) 和美国 (AB890374) 分离的其他雪貂 HEV 具有 82% 和 89% 的核苷酸序列同一性，表明这些毒株形成了一个新的雪貂 HEV 簇，其基因组取决于其宿主所在区域的不同。通过电子显微镜观察粪便标本中直径约为35 nm、密度为1201 g/cm(3)的颗粒。没有证据表明这些颗粒与细胞膜有关。尿液中并未经常检测到雪貂 HEV RNA，这表明雪貂 HEV 排泄到尿液中并不是 HEV 感染的常见特征。 (C) 2017 Elsevier B.V. 保留所有权利。
A novel cluster of five ferret hepatitis E virus (HEV) strains was detected from nine laboratory ferrets (Mustela putorius faro) imported from a ferret farm in the U.S. Our detection of ferret HEV RNA and anti-HEV antibodies, and alanine aminotransferase (ALT) value assessment indicated that all of the 9 ferrets were infected with ferret HEV, and that the infection exhibited three patterns: sub-clinical infection (n = 2), acute hepatitis (n = 6) and persistent infection (n = 1). Next-generation sequence analyses of the entire genome sequences of the five strains revealed that their nucleotide sequence identities ranged from 99.5% to 99.9%, indicating that genetically similar ferret HEVs had been circulating at this the U.S. ferret farm. In contrast, the strains shared 82% and 89% nucleotide sequence identities with other ferret HEV that isolated from the Netherlands (JN998607) and the U.S. (AB890374), suggesting that these strains form a novel cluster of ferret HEV with diverse genomes depending on the region where their host. Particles with a diameter of similar to 35 nm at a density of 1201 g/cm(3) were observed in the fecal specimens by electron microscopy. There was no evidence that the particles were associated with the cell membrane. The ferret HEV RNA was not constantly detected in urine, suggesting that the excretion of ferret HEV into urine is not a common feature of HEV infection. (C) 2017 Elsevier B.V. All rights reserved.