Preparation and properties of antibody directed specifically against the neuraminidase of influenza virus.
Preparation and properties of antibody directed specifically against the neuraminidase of influenza virus.
复制标题
特异性针对流感病毒神经氨酸酶的抗体的制备和特性。
作者：
R. Webster;W. Laver
制备了一株含A0型血凝素和A2(RI/5+)型神经氨酸酶的重组流感病毒抗血清。该抗血清依次被新城疫病毒和A2病毒吸收，然后针对A2型神经氨酸酶的抗体从A2病毒上解离。
该抗体对A0、A1、A2流感病毒的血凝素活性及A0、A1病毒的感染性均无抑制作用。它能中和A2型流感病毒的酶活性，但不能中和A0型或A1型流感病毒。
抗酶抗体可明显中和含有A2型神经氨酸酶的流感病毒的传染性，前提是该抗体在试验期间与细胞保持接触。这种抗体不能防止敏感细胞的感染，但明显阻止了可检测到的血凝素水平的释放。对缺乏神经氨酸酶活性的A2型重组病毒的感染性无影响。
Antiserum was prepared against a recombinant influenza virus possessing A0 (NWS strain) type hemagglutinin and A2 (RI/5+) type neuraminidase. This antiserum was absorbed sequentially with NWS and A2 viruses and antibody directed against A2 type neuraminidase was then dissociated from the A2 virus.
This antibody had no activity against the hemagglutinin activity of A0, A1 and A2 influenza viruses or against the infectivity of A0 and A1 viruses. It neutralized the enzyme activity of A2 but not A0 or A1 type influenza viruses.
Anti-enzyme antibody caused apparent neutralization of the infectivity of influenza viruses possessing A2 type neuraminidase providing that the antibody was allowed to remain in contact with the cells during the test period. This antibody did not prevent infection of susceptible cells but apparently prevented the release of detectable levels of hemagglutinin. It had no effect on the infectivity of an A2 type recombinant virus that lacked neuraminidase activity.