Effect of solvent removal technique on the matrix characteristics of polylactide/glycolide microspheres for peptide delivery
Effect of solvent removal technique on the matrix characteristics of polylactide/glycolide microspheres for peptide delivery
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DOI：
10.1016/0168-3659(95)00125-5
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发表时间：
1996-02-01
影响因子：
10.8
通讯作者：
DeLuca, PP
中科院分区：
文献类型：
--
作者：
Jeyanthi, R;Thanoo, BC;DeLuca, PP
使用温度梯度（Tmp）或稀释（连续相（CP）的莳萝）通过水乳化工艺制备不同基质特性的多孔鲑鱼降钙素（sCT）负载PLGA微球（尺寸范围35-140μm）。Tmp技术产生具有中空内芯和多孔壁的微球。多孔壁的芯尺寸和厚度为 取决于所使用的温度梯度。温度从 15 摄氏度快速上升到 40 摄氏度会产生较大的核心和较薄的壁，而温度逐渐升高会产生较小的核心。 Dil 技术生产的微球具有均匀的蜂窝状孔结构，无核，孔径取决于所用的稀释体积。 Tmp技术制备的微球比表面积较高，堆积密度较低，但无显着差异。 两种技术之间的肽负载量 (3.2-4.5%)。在 Tmp 技术的情况下观察到 CH2Cl2 的快速去除，而 Dil 技术则促进了较慢且逐渐的 CH2Cl2 去除。残留 CH2Cl2 约为
Porous salmon calcitonin (sCT)-loaded PLGA microspheres (size range 35-140 mu m) of varying matrix characteristics were prepared by an aqueous emulsification process using either a temperature gradient (Tmp) or dilution (Dill of the continuous phase (CP). The Tmp technique resulted in microspheres with a hollow internal core and a porous wall. The core size and thickness of the porous wall were dependent on the temperature gradient used. A rapid ramp of temperature from 15 to 40 degrees C resulted in a large core and a thin wall, while a gradual temperature rise resulted in a smaller core. The Dil technique produced microspheres with a uniform, honeycomb like pore structure without a core, pore size being dependent on the dilution volume used. The specific surface area was higher and bulk density lower for microspheres prepared by the Tmp technique while there was no significant difference in the peptide load (3.2-4.5%) between both techniques. A rapid removal of CH2Cl2 was observed in case of the Tmp technique while the Dil technique facilitated a slower and gradual CH2Cl2 removal. Residual CH2Cl2 was approximately