Ultratrace Analysis of Neomycin Residues in Milk at Femtogram Levels by Flow-Through Immunoaffinity Chromatography Test
Ultratrace Analysis of Neomycin Residues in Milk at Femtogram Levels by Flow-Through Immunoaffinity Chromatography Test
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DOI：
10.1007/s12161-021-02058-5
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发表时间：
2021-06
影响因子：
2.9
通讯作者：
Chen Liu;Yanliang Jiang;Linyun Xiu;Ruijun Qian;Mengxi Zhao;P. Luo;Yuebin Ke;Guangming Li;Wenxiao Jiang
中科院分区：
文献类型：
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作者：
Chen Liu;Yanliang Jiang;Linyun Xiu;Ruijun Qian;Mengxi Zhao;P. Luo;Yuebin Ke;Guangming Li;Wenxiao Jiang
牛奶中新霉素的残留对人体健康具有潜在的危害，目前广泛采用免疫检测法进行新霉素等食品污染残留的现场检测。本文将新霉素B与载体蛋白偶联，制备了特异性高的抗体。基于所制备的抗体，建立了牛奶中新霉素B残留的酶联免疫吸附试验（ELISA）和流动免疫亲和层析试验（FTIACT）。所建立的ELISA检测限为162.5 pg/mL，适用于皮克级新霉素B残留的检测;优化后的FTIACT检测限为326.8 fg/mL，适用于飞克级新霉素B残留的现场检测。对于定量FTIACT，加标牛奶的回收率范围为69%至131%，相对标准偏差小于15%。与传统的ELISA方法相比，新霉素B残留分析的检测限更低。
The neomycin residues in milk could pose potential hazard for human health, and now, immunoassays are widely used for on-site screening of neomycin and other food contaminate residues. In this paper, the neomycin B was conjugated with carrier protein as immunogens, and a highly specific antibody was produced. Based on the produced antibody, an enzyme-linked immunosorbent assay (ELISA) and a new flow-through immunoaffinity chromatography test (FTIACT) were developed for rapid analysis of neomycin B residues in milk. The developed ELISA has a detection limit of 162.5 pg/mL in milk, which is suitable for detecting neomycin B residues at picogram levels, while the optimized FTIACT exhibited satisfactory compatibility with the ELISA, with limits of detection of 326.8 fg/mL in milk, which is more suitable for on-site detecting neomycin B residues at femtogram levels. For the quantitative FTIACT, the recoveries from spiked milk ranged from 69 to 131%, with a relative standard deviation of less than 15%. Compared to traditional ELISA formats, the developed FTIACT provided lower detection limits for neomycin B residue analysis.