Characterization of new mutations in pyrazinamide-resistant strains of Mycobacterium tuberculosis and identification of conserved regions important for the catalytic activity of the pyrazinamidase PncA
Characterization of new mutations in pyrazinamide-resistant strains of Mycobacterium tuberculosis and identification of conserved regions important for the catalytic activity of the pyrazinamidase PncA
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DOI：
10.1128/aac.43.7.1761
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发表时间：
1999-07-01
影响因子：
4.9
通讯作者：
Jarlier, V
中科院分区：
文献类型：
--
作者：
Lemaitre, N;Sougakoff, W;Jarlier, V
在 19 种吡嗪酰胺耐药结核分枝杆菌菌株的 pncA 基因中发现了一组新的突变，包括插入序列 IS6110 的转位。来自结核分枝杆菌的 PncA 蛋白与来自不同细菌物种的同源蛋白的比对揭示了 PncA 中的三个高度保守的区域，这些区域可能在吡嗪酰胺的加工中发挥重要作用。
A new set of mutations, including transposition of the insertion sequence IS6110, was identified in the pncA gene from 19 pyrazinamide-resistant Mycobacterium tuberculosis strains. Alignment of the PncA protein from M. tuberculosis with homologous proteins from different bacterial species revealed three highly conserved regions in PncA which may play an important role in the processing of pyrazinamide.