Ordered DNA−polypeptide complexes of extreme chirality: effects of polypeptide handedness on DNA long-range asymmetry
Ordered DNA−polypeptide complexes of extreme chirality: effects of polypeptide handedness on DNA long-range asymmetry
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极端手性的有序DNA-多肽复合物：多肽旋向性对DNA长程不对称性的影响
DOI：
10.1021/ja00232a043
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
A. Minsky
中科院分区：
文献类型：
--
作者：
S. Weinberger;C. Berman;A. Minsky
在适当的离子强度条件下，在各种聚合物或脱水剂存在下，DNA分子经历了一个显著的协同压缩过程。[1]所得到的结构，由于它们与在体内总是发现的DNA的紧密状态的潜在相关性而引起了人们的极大兴趣，其特征在于非保守圆二色光谱，其幅度明显大于分散的DNA分子。2 CD信号的形状和大小都表明，缩合过程伴随着有序三级结构的形成，在三级结构中，发色团之间产生了明确的长程耦合。特别感兴趣的是当DNA在高离子强度条件下被脱水剂浓缩时观察到的现象。3在35%（v/v）乙醇和0.8M NaCl中，DNA（1）（a）Jordan，C. F.地;勒曼湖S.;维纳布尔，J.H. Nature（伦敦）1972，236，67-70. (b)Gobel，LC; Shellman，J. A. Nature（伦敦）1976，259，333-335. (c)郑，S- M.; Mohr，SC Biopolymers 1975，14，663 -674. (d)莱姆利湾K. Proc. Natl. Acad. Sci. USA 1975，72，4288-4292. (e)Yevdokimov，Y. M.;萨利亚诺夫Nucleic Acid Res.1981，9，743-752. (f)Shin，YA; Eichhorn，GL Biopolymers 1984，23，325-335.（2）（a）Shin，YA; Eichhorn，G. L. Biopolymers 1977，16，225-230. (b)赖希，C.; Maestre，M. F.地; Edmondson，S.;格雷，D. M.生物化学1980，19，5208-5213。(c)Maestre，M. F.地;赖希角生物化学1980，19，5214-5223。(d)Keller，D.; Bustamante，CJ Chem.Phys.1986，84，2972-2980.
Under appropriate conditions of ionic strengthand in the presence of various polymers or dehydrating agents, DNA mol-ecules undergo a remarkable cooperative compaction process. 1 The resulting structures, which attracted much interest due to their potentialrelevance to compact states of DNA invariably found in vivo, are characterized by nonconservative circular dichroism spectra whose magnitudes are significantly larger than those of dispersed DNA molecules. 2 Both theshape and the size of the CD signals indicate that the condensation process is ac-companied by the formation of ordered tertiary structures in which well-defined, long-range couplings between chromophores are induced. Of particular interest are the phenomena observed when the DNA is condensedby dehydrating agents at high ionic strength conditions. 3 In 35%(v/v) ethanol and 0.8 M NaCl the DNA (1)(a) Jordan, C. F.; Lerman, L. S.; Venable, J. H. Nature (London) 1972, 236, 67-70.(b) Gosule, LC; Shellman, J. A. Nature (London) 1976, 259, 333-335.(c) Cheng, S.-M.; Mohr, SC Biopolymers 1975, 14,663-674.(d) Laemmli, U. K. Proc. Natl. Acad. Sci. USA 1975, 72, 4288-4292.(e) Yevdokimov, Y. M.; Salyanov, V. I. Nucleic Acid Res. 1981, 9, 743-752.(f) Shin, YA; Eichhorn, GL Biopolymers 1984, 23, 325-335.(2)(a) Shin, YA; Eichhorn, G. L. Biopolymers 1977, 16, 225-230.(b) Reich, C.; Maestre, M. F.; Edmondson, S.; Gray, D. M. Biochemistry 1980, 19, 5208-5213.(c) Maestre, M. F.; Reich, C. Biochemistry 1980, 19, 5214-5223.(d) Keller, D.; Bustamante, CJ Chem. Phys. 1986, 84, 2972-2980.