Root-specific expression of genes for novel glycine-rich proteins cloned by use of an antiserum against xylem sap proteins of cucumber.
Root-specific expression of genes for novel glycine-rich proteins cloned by use of an antiserum against xylem sap proteins of cucumber.
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DOI：
10.1093/oxfordjournals.pcp.a029338
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发表时间：
1998-12
影响因子：
4.9
通讯作者：
C. Sakuta;A. Oda;S. Yamakawa;S. Satoh
中科院分区：
文献类型：
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作者：
C. Sakuta;A. Oda;S. Yamakawa;S. Satoh
利用黄瓜根的poly(a)+ RNA和黄瓜XSPs抗血清对木质部汁液蛋白（xylem sap proteins, XSPs）进行cDNA文库筛选，分离出候选的XSPs cDNA。检测了相应转录本的表达模式和cdna序列。在每个推断的氨基酸序列中，选择两个富含甘氨酸结构域且转录本主要在根中表达的cdna进行进一步分析。这两种cdna编码的蛋白质具有假定的信号序列，在编码多肽的氨基端具有相似的非甘氨酸丰富结构域。相应的mrna在黄瓜下胚轴扦插形成不定根和维管组织发育的过程中同时积累，且在主根的根毛区有较强的表达。这些数据表明，这两种新的富含甘氨酸的蛋白质（被认为是XSPs）的产生可能与根中维管组织的功能有关。
Candidate cDNAs for xylem sap proteins (XSPs) were isolated by screening of a cucumber cDNA library prepared from poly(A)+ RNA of cucumber roots with an antiserum raised against the XSPs of cucumber. The patterns of expression of corresponding transcripts and the sequences of the cDNAs were examined. Two cDNAs with a glycine-rich domain in each deduced amino acid sequence and whose transcripts were expressed predominantly in roots were selected for further analysis. Both cDNAs encoded proteins with a putative signal sequence, with similar non-glycine-rich domains at the amino terminal of the encoded polypeptides. The corresponding mRNAs accumulated concurrently with the formation of adventitious roots from cuttings of cucumber hypocotyls and the development of vascular tissues, and strong expression of the mRNAs was detected in the root-hair zone of tap roots. These data suggest that the production of the two novel glycine-rich proteins, which are putative XSPs, might be associated with the functions of the vascular tissues in roots.