Isolation and sequence of a cDNA clone for the rat pulmonary surfactant-associated protein (PSP-A).
Isolation and sequence of a cDNA clone for the rat pulmonary surfactant-associated protein (PSP-A).
复制标题
大鼠肺表面活性物质相关蛋白 (PSP-A) cDNA 克隆的分离和测序。
DOI：
10.1016/s0006-291x(87)80519-3
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发表时间：
1987
影响因子：
3.1
通讯作者：
Voelker,D
中科院分区：
文献类型：
--
作者：
Sano,K;Fisher,J;Mason,RJ;Kuroki,Y;Schilling,J;Benson,B;Voelker,D
肺表面活性剂主要由磷脂和两组载脂蛋白组成。其中一种载脂蛋白是糖蛋白家族（肺表面活性剂相关蛋白a， PSP-A）。我们分离并测序了一个编码大鼠PSP-A的cDNA克隆，并从其核苷酸序列推导出了完整的氨基酸序列。对经二氧化硅处理的大鼠PSP-A的n端56个氨基酸序列进行了独立测定，与cDNA推断的序列完全一致。分离的大鼠肺泡II型细胞含有两种表达该蛋白的mRNA。
Pulmonary surfactant is composed mainly of phospholipid and two groups of apoproteins. One of these apoproteins is a family of glycoproteins (pulmonary surfactant-associated protein A, PSP-A). We have isolated and sequenced a cDNA clone encoding for rat PSP-A and the full amino acid sequence has been deduced from the nucleotide sequence. The sequence of 56 amino acids at the N-terminus of PSP-A isolated from rats treated with silica was determined independently, and there is complete agreement with the sequence deduced from the cDNA. Isolated rat alveolar type II cells contain two species of mRNA for this protein.