O-Antigens of Escherichia coli Strains O81 and HS3-104 Are Structurally and Genetically Related, Except O-Antigen Glucosylation in E. coli HS3-104
O-Antigens of Escherichia coli Strains O81 and HS3-104 Are Structurally and Genetically Related, Except O-Antigen Glucosylation in E. coli HS3-104
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DOI：
10.1134/s0006297918050061
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发表时间：
2018-05
期刊：
影响因子：
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通讯作者：
E. Zdorovenko;Yu Wang;A. Shashkov;T. Chen;O. Ovchinnikova;Bin Liu;A. Golomidova;Vladislav V. Babenko;Andrey V. Letarov;Y. Knirel
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文献类型：
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作者：
E. Zdorovenko;Yu Wang;A. Shashkov;T. Chen;O. Ovchinnikova;Bin Liu;A. Golomidova;Vladislav V. Babenko;Andrey V. Letarov;Y. Knirel
以大肠杆菌O 81和E.大肠杆菌HS 3 -104。通过单糖分析、一维和二维1H-和13 C-NMR谱研究了O 81 OPS菌株经48%HF处理后的寡糖和OPS的结构。两种OPS具有相似的结构，不同之处仅在于菌株HS 3 -104 OPS中存在侧链葡萄糖残基。两种菌株中O-抗原生物合成基因簇的基因和组织几乎相同，除了菌株HS 3 -104中负责葡萄糖基化的gtr基因簇，其位于基因组的其他位置。
Glycerophosphate-containing O-specific polysaccharides (OPSs) were obtained by mild acidic degradation of lipopolysaccharides isolated fromEscherichia colitype strain O81 andE. colistrain HS3-104 from horse feces. The structures of both OPSs and of the oligosaccharide derived from the strain O81 OPS by treatment with 48% HF were studied by monosaccharide analysis and one- and two-dimensional1H- and13C-NMR spectroscopy. Both OPSs had similar structures and differed only in the presence of a side-chain glucose residue in the strain HS3-104 OPS. The genes and the organization of the O-antigen biosynthesis gene cluster in both strains are almost identical with the exception of thegtrgene cluster responsible for glucosylations in the strain HS3-104, which is located elsewhere in the genome.