EFFECTS OF SYNTHETIC AND NATURALLY-OCCURRING FLAVONOIDS ON MITOGEN-INDUCED PROLIFERATION OF HUMAN PERIPHERAL-BLOOD LYMPHOCYTES
EFFECTS OF SYNTHETIC AND NATURALLY-OCCURRING FLAVONOIDS ON MITOGEN-INDUCED PROLIFERATION OF HUMAN PERIPHERAL-BLOOD LYMPHOCYTES
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DOI：
10.1016/0024-3205(89)90028-3
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发表时间：
1989-01-01
期刊：
影响因子：
6.1
通讯作者：
AKIBA, M
中科院分区：
文献类型：
--
作者：
HIRANO, T;OKA, K;AKIBA, M
在伴刀豆球蛋白A作为有丝分裂原的存在下，对17种合成和天然存在的黄酮类化合物对人淋巴细胞增殖的影响进行了检查。12个黄酮类化合物检查是单羟基或甲氧基衍生物。从体外[3 H]胸苷掺入细胞的程度评价淋巴细胞的促分裂素诱导的反应。所有的化合物都显示出抑制作用; 4.5-77.7%的[3 H]胸苷掺入被1.0 μ g/ml浓度阻断。通过染料排除试验评估的治疗前后淋巴细胞的活力表明没有变化，因此类黄酮可能抑制DNA合成。具有5-羟基、5-甲氧基和6-甲氧基的黄酮类化合物以及具有环己基取代苯基取代基的黄酮类化合物（即2-环己基苯并吡喃-4-酮）表现出最大的抑制作用。任何一种药物的抑制作用均不及泼尼松龙的一半，但基本上与布雷定宁或硫唑嘌呤相当或稍强。因此，似乎众所周知的类黄酮的抗炎作用可能部分来自于抑制淋巴细胞的增殖反应。
Examination was made of the effects of 17 synthetic and naturally occurring flavonoids on human lymphocyte proliferation in the presence of concanavalin A as a mitogen. Twelve of the flavonoids examined were mono-hydroxy or methoxy derivatives. The mitogen-induced response of lymphocytes was evaluated from the extent of the incorporation of [3H]thymidine into cells in vitro. All the compounds showed inhibitory effects; 4.5-77.7% of [3H]thymidine incorporation was blocked by an 1.0 .mu.g/ml concentration. The viability of lymphocytes before and after treatment, as assessed by a dye exclusion test, indicated no change, and thus the flavonoids may inhibit DNA synthesis. The flavonoids possessing 5-hydroxyl, 5-methoxyl and 6-methoxyl group, and those with cyclohexyl instead of phenyl substituent (i.e. 2-cyclohexyl-benzopyran-4-one), showed the greatest inhibition. The inhibitory effect of any one of them was less than one half that of prednisolone, but essentially the same or somewhat exceeding tht of bredinine or azathioprine. It would thus appear that the well-known anti-inflammatory effects of flavonoids may possibly arise in part from the inhibition of the proliferative response of lymphocytes.