CircFOXO3 functions as a molecular sponge for miR-143-3p to promote the progression of gastric carcinoma via upregulating USP44
CircFOXO3 functions as a molecular sponge for miR-143-3p to promote the progression of gastric carcinoma via upregulating USP44
复制标题
CircFOXO3作为miR-143-3p的分子海绵，通过上调USP44促进胃癌的进展
DOI：
10.1016/j.gene.2020.144798
复制
发表时间：
2020-08-30
期刊：
影响因子：
3.5
通讯作者：
Liu, Gao
中科院分区：
文献类型：
--
作者：
Xiang, Tian;Jiang, Hong-sheng;Liu, Gao
胃癌（GC）在全球癌症发病率中排名第五，在癌症相关死亡中排名第三，并造成巨大的健康和经济负担。 GC 形成和进展的分子机制仍不清楚。我们的目的是确定环状 RNA circFOXO3 在 GC 中的参与，并确定其潜在机制。在这项研究中，我们揭示了环状 RNA circFOXO3 在体内肿瘤生长中的刺激作用。 CircFOXO3在体外增强GC细胞增殖和迁移，在体内促进GC细胞肿瘤生长。生物信息学分析表明，circFOXO3 可能通过特异性结合 microRNA (miR)-143-3p 来调节 USP44 表达。通过 RNA 结合蛋白免疫沉淀、荧光素酶报告基因测定和 RNA Pull-down 实验证实了 GC 细胞中 circFOXO3-miR-143-3p-USP44 轴的存在。所有数据表明，circFOXO3 通过靶向 miR-143-3p 上调 USP44 表达来促进 GC 细胞增殖和迁移。
Gastric carcinoma (GC) ranks fifth in terms of cancer morbidity and third in cancer-related death worldwide and imposes enormous health and economic burdens. The molecular mechanisms underlying GC formation and progression remain unclear. Our aim was to identify the involvement of circular RNA circFOXO3 in GC, and to determine the underlying mechanisms. In this study, we revealed a stimulatory role of circular RNA circFOXO3 in tumor growth in vivo. CircFOXO3 enhanced GC cell proliferation and migration in vitro and promoted tumor growth of GC cells in vivo. Bioinformatic analysis revealed that circFOXO3 might regulate USP44 expression by specifically binding to microRNA (miR)-143-3p. Existence of circFOXO3-miR-143-3p-USP44 axis in GC cells was confirmed by RNA-binding protein immunoprecipitation, luciferase reporter assay, and an RNA pull-down experiments. All the data indicate that circFOXO3 promotes GC cell proliferation and migration by upregulating USP44 expression via targeting of miR-143-3p.