Disrupted iron metabolism in peritoneal fluid may induce oxidative stress in the peritoneal cavity of women with endometriosis
Disrupted iron metabolism in peritoneal fluid may induce oxidative stress in the peritoneal cavity of women with endometriosis
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DOI：
10.26444/aaem/75802
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发表时间：
2018-01-01
影响因子：
1.7
通讯作者：
Kotarski, Jan
中科院分区：
文献类型：
--
作者：
Polak, Grzegorz;Barczynski, Barttomiej;Kotarski, Jan
简介和目标。关于腹腔液自由基介导的氧化损伤在子宫内膜异位症发病机制中可能发挥的作用的数据仍然不一致。本研究的目的是确定子宫内膜异位症女性腹腔液中铁代谢标志物及其对氧化应激参数的影响。材料和方法。该研究纳入了 110 名患有子宫内膜异位症的女性和 119 名患有良性卵巢囊肿的患者。在腹腔镜检查过程中，从前侧和后侧的死胡同中吸出所有可见的腹膜液。直视下进行，避免血液污染。使用标准比色试剂盒测量血红蛋白、铁、总氧化状态和总抗氧化状态。结果。与参考组相比，子宫内膜异位症患者腹腔液中的血红蛋白、铁水平以及总氧化状态值显着较高，而总抗氧化状态值显着较低。与月经周期阶段相关的所有测量参数的腹膜液浓度没有观察到差异。结论。患有子宫内膜异位症的女性腹腔液的特点是铁代谢紊乱。这很可能与子宫内膜异位女性腹腔内红细胞数量增加有关，从而导致该环境中血红蛋白浓度较高。铁稳态受损可能通过血红蛋白衍生物的直接影响和/或促炎和促氧化环境的形成对腹膜子宫内膜异位症的病理生理学产生重大影响。腹膜腔氧化应激主要发生在疾病晚期的女性中。
Introduction and objective. Data on the possible role of peritoneal fluid free radical-mediated oxidative damage in the pathogenesis of endometriosis still remains inconsistent. The aim of the study was to determine iron metabolism markers and their influence on oxidative stress parameters in the peritoneal fluid of women with endometriosis.Materials and method. 110 women with endometriosis and 119 patients with benign ovarian cysts were included in the study. All visible peritoneal fluid was aspirated during laparoscopy from the anterior and posterior cul-de-sacs. under direct vision to avoid blood contamination. Haemoglobin, iron, total oxidative status, and total antioxidant status were measured using standard colourimetric kits.Results. Haemoglobin, iron levels, as well as total oxidative status values were significantly higher, whereas total antioxidant status values were significantly lower in the peritoneal fluid of patients with endometriosis, in comparison to the reference groups. No differences were observed in peritoneal fluid concentrations of all parameters measured in relation to the phase of the menstrual cycle.Conclusions. Peritoneal fluid of women with endometriosis is characterized by disrupted iron metabolism. This is most likely related to an increased number of erythrocytes in the peritoneal cavity of endometriotic women, which leads to a higher concentration of haemoglobin in this environment. Impaired iron homeostasis may have a significant influence on the pathophysiology of peritoneal endometriosis by the direct impact of haemoglobin derivatives and/or formation of the pro-inflammatory and pro-oxidative environment. Peritoneal cavity oxidative stress occurs predominantly in women in advanced stages of the disease.