Vibrational imaging based on stimulated Raman scattering microscopy
Vibrational imaging based on stimulated Raman scattering microscopy
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DOI：
10.1088/1367-2630/11/3/033026
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发表时间：
2009-03-25
影响因子：
3.3
通讯作者：
Volkmer, A.
中科院分区：
文献类型：
--
作者：
Nandakumar, P.;Kovalev, A.;Volkmer, A.
利用高重复频率（76 MHz）的近红外皮秒激光脉冲和射频锁定检测，实现了受激拉曼散射显微镜。基于受激拉曼损失检测，我们展示了非侵入性的逐点振动映射的化学和生物样品具有高灵敏度，而不需要标记的样品与天然或人工荧光团。我们实验证明了这种技术的一个主要好处，这是专门响应的线性拉曼共振性质的样品的能力，从而允许直接定量解释的拉曼活性模式的数量密度方面的图像对比度。
A stimulated Raman scattering microscope with near-infrared picosecond laser pulses at high repetition rates (76 MHz) and radio-frequency lock-in detection is accomplished. Based on stimulated Raman loss detection, we demonstrate noninvasive point-by-point vibrational mapping of chemical and biological samples with high sensitivity and without the requirement for labeling of the sample with natural or artificial fluorophores. We experimentally demonstrate a major benefit of this technique, which is the capability to respond exclusively to the linear Raman-resonance properties of the sample, thus allowing a direct quantitative interpretation of image contrast in terms of the number density of Raman-active modes.