A comparison of the polypeptides of human and bovine respiratory syncytial viruses and murine pneumonia virus.
A comparison of the polypeptides of human and bovine respiratory syncytial viruses and murine pneumonia virus.
复制标题
人、牛呼吸道合胞病毒和鼠肺炎病毒多肽的比较。
DOI：
10.1016/0042-6822(77)90012-5
复制
发表时间：
1977
期刊：
影响因子：
3.7
通讯作者：
C. Pringle
中科院分区：
文献类型：
--
作者：
P. Cash;W. Wunner;C. Pringle
人和牛呼吸道合胞病毒(RS)和小鼠肺炎病毒(PVM)是形态相似的病毒，是偏粘病毒(或肺炎病毒)组中唯一已知的成员。用聚丙烯酰胺凝胶电泳法(PAGE)对这些病毒的多肽进行了比较，结果表明，在血清学上相关的人和。牛RS病毒具有相似的多肽谱。然而，PVM与其他两种病毒并不十分相似。RS病毒多肽的分子量是通过不连续的SDS-PAGE在梯度平板凝胶中确定的，除了检测到额外的10,000 mW的多肽外，与以前用连续的SDS-PAGE(Wunner和Pringle，1976)测定的结果相当。对来自不同地区或同一地点不同时间分离的11株人RS病毒的比较表明，VP32(一种功能未知的非糖基化多肽)的相对迁移率是每个毒株的稳定特征。其他病毒多肽的迁移率变化不大。这11个菌株分为三个组，其中VP32的分子量估计为31,000，32,000和35,000，但与起源日期或地点没有明显的相关性。
Human and bovine respiratory syncytial (RS) viruses and pneumonia virus of mice (PVM) are morphologically similar viruses and are the only known members of the metamyxovirus (or pneumovirus) group. Comparison of the polypeptides of these viruses by polyacrylamide-gel electrophoresis (PAGE) has shown that the serologically related human and. bovine RS viruses have similar polypeptide profiles. However, PVM does not resemble the other two viruses closely. The molecular weights of RS virus polypeptides were established by discontinuous SDS-PAGE in gradient slab gels and were comparable to previous determinations by continuous SDS-PAGE (Wunner and Pringle, 1976), apart from the detection of an additional 10,000-MW polypeptide. Comparison of 11 strains of human RS virus isolated from different localities, or from the same locality at different dates, showed that the relative mobility of VP32 (a nonglycosylated polypeptide of unknown function) was a stable characteristic of each strain. The mobilities of the other viral polypeptides showed little variation. The 11 strains fell into three groups in which the molecular weights of VP32 were estimated to be 31,000, 32,000, and 35,000, but there was no clear correlation with date or place of origin.