Signal turn-on probe for nucleic acid detection based on 19F nuclear magnetic resonance
Signal turn-on probe for nucleic acid detection based on 19F nuclear magnetic resonance
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DOI：
10.1016/j.bmcl.2010.11.013
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发表时间：
2011-01-01
影响因子：
2.7
通讯作者：
Fujimoto, Kenzo
中科院分区：
文献类型：
--
作者：
Sakamoto, Takashi;Shimizu, Yu-ki;Fujimoto, Kenzo
为了对体内基因表达进行成像，我们设计并合成了一种基于顺磁弛豫增强的新型F-19核磁共振（MR）信号开启探针。将具有点突变K-ras mRNA的分子信标序列的茎环结构的寡脱氧核糖核苷酸（ODN）分别用双（三氟甲基）苯部分和Gd-1，4，7，10-四氮杂环十二烷-1，4，7，10-四乙酸螯合物部分在ODN探针的每个末端进行双重标记。我们发现，通过添加互补ODN，连接在探针5'末端的双（三氟甲基）苯部分的F-19 MR信号被打开。该探针具有在体内成像基因表达的潜力。(C)2010爱思唯尔有限公司保留所有权利。
To image gene expression in vivo, we designed and synthesized a novel signal turn-on probe for F-19 nuclear magnetic resonance (MR) imaging based on paramagnetic relaxation enhancement. The stem-loop structured oligodeoxyribonucleotide (ODN) having a molecular beacon sequence for point mutated K-ras mRNA was doubly labeled with bis(trifluoromethyl)benzene moiety and Gd-1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid chelate moiety at the each termini of the ODN probe, respectively. We found that the F-19 MR signal of the bis(trifluoromethyl) benzene moiety tethered at the 5' termini of the probe turned on by the addition of complementary ODN. The probe has the potential to image gene expressions in vivo. (C) 2010 Elsevier Ltd. All rights reserved.