Susceptibility of spotted doves (Streptopelia chinensis) to experimental infection with the severe fever with thrombocytopenia syndrome phlebovirus
Susceptibility of spotted doves (Streptopelia chinensis) to experimental infection with the severe fever with thrombocytopenia syndrome phlebovirus
复制标题
中华斑鸠（Streptopelia chinensis）实验性感染血小板减少综合征高热白斑病毒的易感性
DOI：
10.1371/journal.pntd.0006982
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发表时间：
2019-07-01
影响因子：
3.8
通讯作者：
Xing, Zheng
中科院分区：
文献类型：
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作者：
Li, Zhifeng;Bao, Changjun;Xing, Zheng
背景 严重发热血小板减少综合征病毒 (SFTSV) 是一种通过蜱虫自然传播的新出现的人类病原体，自 2010 年首次发现以来已广泛传播。尽管已在野生鸟类中检测到 SFTSV 特异性抗体，但这些病毒的天然储存宿主和扩增宿主尚未得到充分研究。方法/原理发现在这里，我们报告了两种SFTSV毒株对斑鸠（中国链球菌）的实验性感染，即JS2010-14（中国谱系毒株）和JS2014-16（日本谱系毒株），它们代表了目前在东亚流行的主要病毒基因型。在这些研究中，我们确定斑点鸽子对SFTSV敏感，并且病毒血症的严重程度与剂量相关。当受到 107 和 105 PFU 的攻击时，所有鸽子均出现病毒血症，并在感染后 3-5 天 (dpi) 达到高峰。当受到 103 PFU 的攻击时，只有一部分 (25-62.5%) 的鸟类出现病毒血症。 SFTSV 对斑鸠的毒力具有毒株依赖性。实验接种的禽类感染 107 PFU 的 JS2014-16 菌株后，6.8 天内死亡率为 12.5%，平均峰值病毒滴度为 106.9 PFU/mL。所有接种 107 PFU JS2010-14 毒株的鸽子均在 6.1 天内存活下来，平均病毒血症滴度相对较低（峰值为 105.6 PFU/mL）。结论/意义我们的研究结果表明，斑鸠是中国最丰富的鸟类之一，可能是SFTSV的有效扩增宿主，并在其生态中发挥重要作用。在这两种 SFTSV 毒株中，日本毒株导致死亡，受感染禽类的病毒血症滴度比中国毒株在更长的时间内更高。我们的观察揭示了SFTSV的生态，这可能有利于监测和控制计划的实施。
Background Severe fever with thrombocytopenia syndrome virus (SFTSV), an emerging human pathogen naturally transmitted by ticks, has spread widely since it was first detected in 2010. Although SFTSV-specific antibodies have been detected in wild birds, these natural reservoir and amplifying hosts for the virus have not been well studied. Methodology/Principle findings Here we report an experimental infection of spotted doves (Streptopelia chinensis) with two strains of SFTSV, JS2010-14, a Chinese lineage strain, and JS2014-16, a Japanese lineage strain, which represent the main viral genotypes currently circulating in East Asia. In these studies, we have determined that spotted doves are susceptible to SFTSV and the severity of the viremia is dose-dependent. When challenged with 107 and 105 PFU, all doves developed viremia which peaked 3–5 days post infection (dpi). Only a subset (25–62.5%) of the birds developed viremia when challenged at 103 PFU. Virulence of SFTSV in spotted doves was strain dependent. Infection with 107 PFU of strain JS2014-16 resulted in 12.5% mortality over 6.8 days and mean peak viremia titers of 106.9 PFU/mL in experimentally inoculated birds. All doves inoculated with 107 PFU of the JS2010-14 strain survived infection with relatively lower mean viremia titers (105.6 PFU/mL at peak) over 6.1 days. Conclusions/Significance Our results suggest that spotted doves, one of the most abundant bird species in China, could be a competent amplifying host for SFTSV and play an important role in its ecology. Between the two SFTSV strains, the strain of the Japanese lineage caused mortality, higher viremia titers in infected birds over a longer time period than did the Chinese strain. Our observations shed light on the ecology of SFTSV, which could benefit the implementation of surveillance and control programs.