Maintenance of hemiround colonies and undifferentiated state of mouse induced pluripotent stem cells on carbon nanotube-coated dishes
Maintenance of hemiround colonies and undifferentiated state of mouse induced pluripotent stem cells on carbon nanotube-coated dishes
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DOI：
10.1016/j.carbon.2011.01.061
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发表时间：
2011-06-01
期刊：
影响因子：
10.9
通讯作者：
Watari, Fumio
中科院分区：
文献类型：
--
作者：
Akasaka, Tsukasa;Yokoyama, Atsuro;Watari, Fumio
诱导多能干细胞（iPS）已经引起了全世界的广泛关注。然而，只有少数研究研究了有效的培养基质，以维持iPS细胞的无饲料培养。本研究将小鼠iPS细胞在无饲料条件下培养在碳纳米管（CNT）包被培养皿上，观察细胞在培养皿上的集落形态和分化状态。在含有15%胎牛血清（FBS）和白血病抑制因子（LIF）的培养基中培养5 d后，观察到多壁碳纳米管（MWCNTs）厚膜上的菌落呈半圆形；此外，细胞表达早期未分化标志物。另一方面，聚苯乙烯培养皿和胶原包被聚苯乙烯培养皿上的菌落轮廓模糊，扩散良好，大多数扩散细胞仅弱表达早期未分化标记。这些结果表明，MWCNTs厚膜可以维持无饲料条件下培养的小鼠iPS细胞的半圆形集落和未分化状态。(C) 2011 Elsevier Ltd.版权所有。
Induced pluripotent stem (iPS) cells have attracted worldwide interest. However, there have been only a few studies investigating effective culture substrates for feeder-free culturing for the maintenance of iPS cells. In this study, we cultured mouse iPS cells under feeder-free conditions on carbon nanotube (CNT)-coated dishes and then evaluated the colony morphology and differentiation state of the cells on the dishes. After 5 d of cultivation in a medium containing 15% fetal bovine serum (FBS) and leukemia inhibitory factor (LIF), the colonies on thick films of multi-walled CNTs (MWCNTs) were observed to be hemiround; further, the cells expressed early undifferentiation markers. On the other hand, the colonies on a cell culture polystyrene dish and a collagen-coated polystyrene dish showed indistinct outline and spread well, and most spreading cells only weakly expressed early undifferentiation markers. These results indicate that the thick films of MWCNTs could maintain hemiround colonies and undifferentiated state of mouse iPS cells cultured under feeder-free conditions. (C) 2011 Elsevier Ltd. All rights reserved.