Cadherin-8 protein expression in gray matter structures and nerve fibers of the neonatal and adult mouse brain
Cadherin-8 protein expression in gray matter structures and nerve fibers of the neonatal and adult mouse brain
复制标题
DOI：
10.1016/s0306-4522(98)00077-3
复制
发表时间：
1998-11-01
期刊：
影响因子：
3.3
通讯作者：
Ushio, Y
中科院分区：
文献类型：
--
作者：
Korematsu, K;Nishi, T;Ushio, Y
采用兔抗血清免疫组织化学方法研究了新生小鼠和成年小鼠脑内cadherin-8蛋白的拓扑分布。Cadherin-8的表达仅限于新生儿大脑中构成特定神经回路的几个区域，即边缘系统、基底节区-丘脑皮质回路、小脑及相关核。此外，连接部分cadherin-8阳性区域的神经纤维，即束束-脚间束、背被下垂、内侧纵束、桥状横轴纤维、结膜臂和小脑下脚的神经纤维，以及三叉神经脊髓束、动眼神经、面神经和三叉神经的神经纤维均呈cadherin-8阳性。Cadherin-8的表达在上丘中间灰色层呈斑块状分布，分布类似于富含乙酰胆碱酯酶的斑块。在新生儿小脑浦肯野细胞层可见节段组织的cadherin-8阳性区。成年小鼠脑干和小脑中表达cadherin-8的部分细胞核和纤维在新生期也被染色。这些发现表明，钙粘蛋白-8通过将表达该分子的区域与阳性神经纤维连接起来，参与了特定神经回路的形成，并表明其可能与上丘和小脑的亚分区组织有关。(c) 1998 ibro。Elsevier Science Ltd.出版。
The topological distribution of mouse cadherin-8 protein in the neonatal and adult mouse brain was studied immunohistochemically using a rabbit antiserum. Cadherin-8 expression was restricted to several areas in neonatal brains constituting particular neural circuits, i.e. the limbic system, the basal ganglia-thalamocortical circuit, and the cerebellum and related nuclei. In addition, the nerve fibers linking some of the cadherin-8-positive areas, i.e. the habenulo-interpeduncular tract, decussation of the dorsal tegmentum, the medial longitudinal fasciculus, transverse pontine fibers, the brachium conjunctivum and the inferior cerebellar peduncle were cadherin-8 positive, as were the spinal tract of the trigeminal nerve, oculomotor nerve, facial nerve and trigeminal nerve. Cadherin-8 expression also showed a patch-like distribution in the intermediate gray layer of the superior colliculus, resembling acetylcholinesterase-rich patches in allocation. Segmentally organized cadherin-8-positive areas were found in the neonatal cerebellar Purkinje cell layer. Some nuclei and fibers in the brainstem and cerebellum expressing cadherin-8 at neonatal stages, were also stained in the adult mouse brain.These findings suggest that cadherin-8 is involved in the formation of particular neural circuits by connecting areas expressing this molecule with positive nerve fibers, and indicate its possible implication in subdivisional organization in the superior colliculus and cerebellum. (C) 1998 IBRO. Published by Elsevier Science Ltd.