Potential role of Hsp90 in rat islet function under the condition of high glucose
Potential role of Hsp90 in rat islet function under the condition of high glucose
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DOI：
10.1007/s00592-016-0852-2
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发表时间：
2016-03
影响因子：
3.8
通讯作者：
Xue Yang;Yuqing Zhang;Wan Xu;Ruyuan Deng;Yun Liu;Feng-ying Li;Yao Wang;Xueying Ji;Mengyao Bai;Feiye Zhou;Libin Zhou;Xiao Wang
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文献类型：
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作者：
Xue Yang;Yuqing Zhang;Wan Xu;Ruyuan Deng;Yun Liu;Feng-ying Li;Yao Wang;Xueying Ji;Mengyao Bai;Feiye Zhou;Libin Zhou;Xiao Wang
目的保护胰岛β细胞功能是2型糖尿病治疗的关键。大量证据表明热休克蛋白90（Heat-shockprotein 90，Hsp 90）是客户蛋白稳定和正确折叠所必需的，在多种生物过程中起着重要作用。方法分离雄性SD大鼠胰岛，进行RT-PCR、Western blot和胰岛素分泌试验，观察高糖对胰岛β细胞功能的影响。结果高糖暴露后，Hsp 90的表达明显降低，Hsp 90参与葡萄糖激酶的激活和葡萄糖代谢。而不是钙信号。两种Hsp 90抑制剂17-DMAG和CCT 018159均能显著增强葡萄糖刺激的大鼠胰岛胰岛素分泌，同时沿着与胰岛β细胞功能密切相关的基因表达增加。
AimsThe preservation of pancreatic β-cell function is a key point in the treatment of type 2 diabetes mellitus. There is substantial evidence demonstrating that heat-shock protein 90 (Hsp90) is needed for the stabilization and correct folding of client proteins and plays important roles in various biological processes. Here, we revealed the important role of Hsp90 in β-cell function.MethodsIslets from male Sprague–Dawley rats were isolated to be used for further RT-PCR, Western blot, and insulin secretion test ex vivo in response to different stimuli.ResultsOur results revealed that Hsp90 expression was significantly decreased in isolated rat islets exposed to high glucose, which was involved in glucokinase activation and glucose metabolism, not calcium signaling. Two kinds of Hsp90 inhibitors 17-DMAG and CCT018159 markedly enhanced glucose-stimulated insulin secretion from rat islets, along with increased expressions of genes closely related to β-cell function.ConclusionsThese data indicate that Hsp90 may be involved in high glucose-induced islet function adaptation.