DNA replication in vertebrates requires a homolog of the Cdc7 protein kinase.
DNA replication in vertebrates requires a homolog of the Cdc7 protein kinase.
复制标题
脊椎动物中的 DNA 复制需要 Cdc7 蛋白激酶的同源物。
DOI：
10.1073/pnas.96.6.2800
复制
发表时间：
1999
影响因子：
11.1
通讯作者：
Maller,JL
中科院分区：
文献类型：
--
作者：
Roberts,BT;Ying,CY;Gautier,J;Maller,JL
cdc7是酿酒酵母菌DNA复制所必需的基因。最近在脊椎动物中发现了Cdc7p同源物，但它们在DNA复制中的作用尚未得到解决。本研究表明，针对非洲爪蟾同源物xCdc7的抗体会干扰胚胎发育过程中的DNA复制和卵细胞循环提取物中的卵细胞复制。我们还证明了xCdc7与Mcm复合物的细胞周期依赖性关联，Mcm复合物与复制起点结合，也是DNA合成所必需的。综上所述，这些数据表明xCdc7的功能从真菌到脊椎动物都是保守的。xCdc7蛋白在黄体酮刺激静息卵母细胞后积累，这为先前的观察提供了分子解释，即DNA复制能力的发展需要在减数分裂I后期合成蛋白质。
CDC7is an essential gene required for DNA replication inSaccharomyces cerevisiae. Cdc7p homologs have recently been identified in vertebrates, but their role in DNA replication has not yet been addressed. Here we show that antibodies to theXenopus laevishomolog, xCdc7, interfere with DNA replicationin vivoin developing embryos andin vitroin cycling egg extracts. We also demonstrate cell cycle-dependent association of xCdc7 with the Mcm complex, which binds to replication origins and also is required for DNA synthesis. Taken together, these data indicate that the function of xCdc7 is conserved from fungi to vertebrates. xCdc7 protein accumulates after stimulation of resting oocytes with progesterone, suggesting a molecular explanation for previous observations that the development of the capacity for DNA replication requires protein synthesis late in meiosis I.