Comparison of the central and renal dopamine-1 receptor.
Comparison of the central and renal dopamine-1 receptor.
复制标题
中枢和肾脏多巴胺-1 受体的比较。
DOI：
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发表时间：
1990
影响因子：
3.2
通讯作者：
P. Fishman
中科院分区：
文献类型：
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作者：
A. Sidhu;R. Felder;P. Jose;P. Fishman
纹状体膜的多巴胺D-1受体与肾近端小管的多巴胺DA-1受体进行了比较。在用多巴胺-1激动剂SKF R-38393预处理后，用1%胆酸钠和磷脂溶解多巴胺-1受体。去除胆酸钠后，将可溶性受体重组到磷脂囊泡中。使用多巴胺-1拮抗剂[125 I]-SCH 23982通过放射性配体结合研究受体。重建的多巴胺D-1和DA-1受体密度相似。然而，溶解的D-1受体的亲和力比溶解的DA-1受体大17倍。膜结合D-1受体对放射性配体的亲和力也大于膜结合DA-1受体。这种差异的机制仍有待确定。
The dopamine D-1 receptor from striatal membranes was compared with the dopamine DA-1 receptor from renal proximal tubules. The dopamine-1 receptors were solubilized with 1% sodium cholate and phospholipids after pretreatment with the dopamine-1 agonist, SKF R-38393. The soluble receptors were reconstituted into phospholipid vesicles after removal of sodium cholate. The receptors were studied by radioligand binding using the dopamine-1 antagonist [125I]-SCH 23982. The reconstituted dopamine D-1 and DA-1 receptor densities were similar. However, the affinity of the solubilized D-1 receptor was 17-fold greater than the solubilized DA-1 receptor. The affinity of membrane bound D-1 receptor to the radioligand was also greater than that noted for membrane bound DA-1 receptor. The mechanism for this difference remains to be determined.