In Situ Characterizing Membrane Lipid Phenotype of Human Lung Cancer Cell Lines Using Mass Spectrometry Profiling.
In Situ Characterizing Membrane Lipid Phenotype of Human Lung Cancer Cell Lines Using Mass Spectrometry Profiling.
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使用质谱分析原位表征人肺癌细胞系的膜脂质表型
DOI：
10.7150/jca.14310
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发表时间：
2016
影响因子：
3.9
通讯作者：
Li Z
中科院分区：
文献类型：
--
作者：
He M;Guo S;Ren J;Li Z
脂质代谢异常与癌症密切相关。在这项研究中，采用质谱法原位研究了六种人肺癌细胞系（即，A549、H1650、H1975来自腺癌，H157和H1703来自鳞状细胞癌，以及H460来自大细胞癌）与癌细胞类型，最后检测总共230种脂质。基于这230种脂质，偏最小二乘判别分析表明，15种脂质（即，PE 18：0_18：1、PI 18：0_20：4、SM 42：2、PE 16：0_20：4、PE 36：2、PC 36：2、SM 34：1、PA 38：3、C18：0、C22：4、PA 34：2、C20：5、C20：2、C18：2和CerP 36：2）的预测残差平方和（PRESS）评分为0.1974，可以用于区分六种癌细胞系。多不饱和脂肪酸（即，C20：4、C22：4、C22：5和C22：6）和多不饱和磷脂（PE 16：0_20：4、PE 38：4和PI 18：0_20：4）在肺腺癌细胞中观察到，尤其是对于H1975细胞。三种腺癌细胞系（即，A549、H1650和H1975）可以基于C18：1、C20：1、C20：2、C20：5和C22：6的表达与其他肺癌细胞系区分开。
Abnormal lipid metabolisms are closely associated with cancers. In this study, mass spectrometry was employed to in situ investigate the associations of membrane lipid phenotypes of six human lung cancer cell lines (i.e., A549, H1650, H1975 from adenocarcinoma, H157 and H1703 from squamous cell carcinomas, and H460 from a large cell carcinoma) with cancer cell types and finally total 230 lipids were detected. Based these 230 lipids, partial least-square discriminant analysis indicated that fifteen lipids (i.e., PE 18:0_18:1, PI 18:0_20:4, SM 42:2, PE 16:0_20:4, PE 36:2, PC 36:2, SM 34:1, PA 38:3,C18:0, C22:4, PA 34:2, C20:5, C20:2, C18:2, and CerP 36:2) with variable importance in the projection (VIP) value of > 1.0 could be used to differentiate six cancer cell lines with the Predicted Residual Sum of Square (PRESS) score of 0.1974. Positive correlation between polyunsaturated fatty acids (i.e., C20:4, C22:4, C22:5, and C22:6) and polyunsaturated phospholipids (PE 16:0_20:4, PE 38:4, and PI 18:0_20:4) was observed in lung adenocarcinoma cells, especially for H1975 cells. Three adenocarcinoma cell lines (i.e., A549, H1650, and H1975) could be differentiated from other lung cancer cell lines based on the expression of C18:1, C20:1, C20:2, C20:5, and C22:6.