A Microtiter Plate Assay for Sulfonamides
A Microtiter Plate Assay for Sulfonamides
复制标题
磺胺类药物的微量滴定板测定
DOI：
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Timothy J. Smith
中科院分区：
文献类型：
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作者：
S. Tseng;Timothy J. Smith
磺胺甲恶唑、磺胺恶唑和磺胺嘧啶是几种用于治疗感染性疾病的磺胺类药物。一些研究表明，氨基取代基在这些药物的毒性和治疗效果中都起着重要作用。鉴于这些发现，一种快速方便的分析方法将有助于监测选定的接受这些药物的患者。随着临床实验室越来越多地使用微量滴度板方法，基于与氨基取代基的布拉顿-马歇尔反应的测定方法被适应于微量滴度板格式。结果表明，在临床相关浓度的生物液体中，磺胺类药物的微滴板检测保留了必要的灵敏度和线性。该分析方法简单、快速、方便，适用于只需要测量活性药物浓度的监测程序。
Abstract Sulfamethoxazole, sulfisoxazole, and sulfadiazine are sulfonamides used in the treatment of several infectious diseases. Several studies have demonstrated that the amino substituent plays an important role in both the toxicity and the therapeutic effect of these drugs. In view of these findings, a rapid and convenient method of analysis would be useful for monitoring selected patients receiving these drugs. With the increasing use of microtiter plate methodology in the clinical laboratory, an assay based upon the Bratton-Marshall reaction with the amino substituent was adapted to the microtiter plate format. The results indicate that the microtiter plate assay for sulfonamides retains the sensitivity and linearity necessary for analysis of sulfonamides in biological fluids at clinically relevant concentrations. The assay is simple, rapid, convenient, and suitable for monitoring procedures where only the measurement of the active drug concentration is required.
中耳炎的抗菌治疗。
DOI：
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发表时间：
1991
期刊：
Seminars in respiratory infections
影响因子：
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作者：
Giebink,GS;Canafax,DM
通讯作者：
Canafax,DM