Adiponectin mRNA levels in parametrial adipose tissue and serum adiponectin levels are reduced in mice during late pregnancy
Adiponectin mRNA levels in parametrial adipose tissue and serum adiponectin levels are reduced in mice during late pregnancy
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DOI：
10.1055/s-2004-825726
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发表时间：
2004-07-01
影响因子：
2.2
通讯作者：
Toyoda, N
中科院分区：
文献类型：
--
作者：
Kondo, E;Sugiyama, T;Toyoda, N
脂联素是一种脂肪衍生因子，在胰岛素抵抗和肥胖中下调；人类和啮齿类动物在怀孕后期都已证实存在胰岛素抵抗。本研究检查了妊娠期间脂联素基因表达的生理变化以及脂联素的循环水平。我们检查了怀孕小鼠与处女小鼠脂肪组织中产生的脂联素 mRNA 的相对数量。我们还测量了小鼠整个怀孕期间的血清脂联素水平和宫旁脂肪细胞大小。与处女小鼠相比，在怀孕第 15 天和第 18 天，脂联素 mRNA 分别显着减少了 74 +/- 8% 和 63 +/- 4%。妊娠第15天（30.7±8.5μg/ml）和第18天（27.9±8.7μg/ml）血清脂联素浓度下降，且显着低于处女小鼠（56.8±6.6μg/ml）。怀孕第 15 天和第 18 天的小鼠的宫旁脂肪细胞明显大于处女小鼠或怀孕早期的脂肪细胞。脂肪细胞大小与脂联素基因表达的降解和血清脂联素水平密切相关。这些结果表明脂联素表达的变化影响怀孕小鼠的代谢状态。
Adiponectin, a fat-derived factor, is downregulated in insulin resistance and obesity; insulin resistance has been demonstrated during late pregnancy in both humans and in rodents. The present study examines the physiological change of adiponectin gene expression as well as the circulating levels of adiponectin during pregnancy. We examined the relative quantity of adiponectin mRNA produced in the adipose tissues of pregnant compared to virgin mice. We also measured serum adiponectin levels and parametrial adipocyte size in mice throughout pregnancy. Adiponectin mRNA was significantly reduced by 74 +/- 8% and 63 +/- 4 % at days 15 and 18 of pregnancy, respectively, compared to virgin mice. Serum adiponectin concentration decreased on days 15 (30.7 +/- 8.5 mug/ml) and 18 (27.9 +/- 8.7 mug/ml) of pregnancy, and the values were significantly lower than that of virgin mice (56.8 +/- 6.6 pg/ml). Parametrial adipocytes from mice on days 15 and 18 of pregnancy were significantly larger than in virgin mice or during early pregnancy. Fat-cell size was closely correlated to degradation of adiponectin gene expression and serum adiponectin levels. These results suggest that changes of adiponectin expression affect metabolic status in pregnant mice.