Dielectrophoretic Behavior of Single DNA in Planar and Capillary Quadrupole Microelectrodes
Dielectrophoretic Behavior of Single DNA in Planar and Capillary Quadrupole Microelectrodes
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平面和毛细管四极杆微电极中单个 DNA 的介电泳行为
DOI：
10.1246/cl.2001.250
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发表时间：
2001
影响因子：
1.6
通讯作者：
H. Watarai
中科院分区：
文献类型：
--
作者：
S. Tsukahara;K. Yamanaka;H. Watarai
在工作区半径为65 μm的平面四极电极池中，成功地观察到荧光标记的单个DNA（约40 kbp）的介电电泳（DEP）。DEP行为取决于交流电流的频率（f）;在较低的f下为正DEP，在较高的f下为负DEP。DEP力和具有四极电极的毛细管（内径82.5 μm）中的层流的组合控制DNA从毛细管中的洗脱作为f的函数。
Dielectrophoresis (DEP) of single DNA (about 40 kbp) labeled with a fluorophore was successfully observed in a planar quadrupole electrode cell whose working area was 65 µm in radius. The DEP behavior depended on frequency (f) of alternating current; positive DEP at lower f and negative DEP at higher f. The combination of the DEP force and a laminar flow in a capillary (82.5 µm in inner radius) with a quadrupole electrode controlled the elution of DNA from the capillary as a function of f.