Telomere terminal transferase activity in the hypotrichous ciliate Oxytricha nova and a model for replication of the ends of linear DNA molecules.
Telomere terminal transferase activity in the hypotrichous ciliate Oxytricha nova and a model for replication of the ends of linear DNA molecules.
复制标题
次毛纤毛虫 Oxytricha nova 中的端粒末端转移酶活性和线性 DNA 分子末端复制的模型。
DOI：
10.1093/nar/16.14.6953
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发表时间：
1988
影响因子：
14.9
通讯作者：
Prescott,DM
中科院分区：
文献类型：
--
作者：
Zahler,AM;Prescott,DM
我们在植物生长细胞的大核粗提物中发现了丰富的端粒特异性末端转移酶活性。该活性将2 - 7个GGGGTTTT序列的串联重复序列（Oxytrichatelomericrepeat）添加到以G4 T4重复序列结尾的寡核苷酸引物的3′端，并且总是在适当的相位添加重复序列。该活性需要存在微摩尔量的dGTP和dTTP以及末端为3′重复的Oxytrichatelomeric序列的单链寡聚体引物。提取物中存在核酸酶活性，其与端粒末端转移酶活性密切平衡。我们提出了一个简单的模型复制的线性DNA分子的末端的端粒末端转移酶的基础上。
we have found abundant telomere-specific terminal transferase activity in crude macronuclear extracts from vegetatively growing cells of the hypotrichous ciliateOxytrichanovaThis activity adds two to seven tandem repeats of the sequence GGGGTTTT (theOxytrichatelomeric repeat) to the 3′ end of oligonucleotide primers ending in repeats of G4T4and always adds the repeats in the proper phase. The activity requires the presence of micromolar amounts of dGTP and dTTP as well as single-stranded oligomer primers ending 3′ with repeats of theOxytrichatelomeric sequence. A nuclease activity is present in the extracts which is closely balanced with telomere terminal transferase activity. We propose a simple model for replication of the ends of linear DNA molecules based on the telomere terminal transferase.