Use of a swine bioassay and a RT-PCR assay to assess the risk of transmission of swine hepatitis E virus in pigs
Use of a swine bioassay and a RT-PCR assay to assess the risk of transmission of swine hepatitis E virus in pigs
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DOI：
10.1016/s0166-0934(01)00420-7
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发表时间：
2002-03-01
影响因子：
3.1
通讯作者：
Meng, XJ
中科院分区：
文献类型：
--
作者：
Kasorndorkbua, C;Halbur, PG;Meng, XJ
本研究的目的是评估通过接种从实验性感染猪戊型肝炎病毒（猪HEV）的猪身上收集的组织或粪便，将猪戊型肝炎病毒（猪HEV）传播给幼猪的风险。将 75 头 3 周龄的猪随机分为 24 组，每组 3-4 头猪，并接种组织匀浆（肝脏、心脏、胰腺或骨骼肌）或接种后 3、7、14、20、27 或 55 天（DPI）收集的感染 HEV 猪的粪便悬浮液。将每种接种物制备成 PBS 缓冲液中的 10% 悬浮液 (w/v)，并通过猪 HEV RNA 半定量 RT-PCR 和猪生物测定进行测试。肝脏、心脏和胰腺的接种途径为静脉注射，骨骼肌和粪便悬浮液的接种途径为胃管。 RT-PCR 检测显示，在 DPI 3-7 和 14-20 DPI 时收集的肝脏匀浆接种物和粪便对猪 HEV RNA 呈阳性。接种在 DPI 3-7 和 14-20 DPI 收集的肝匀浆的猪产生了抗 HEV 抗体，并且在其血清中检测到了猪 HEV RNA。接种心脏、胰腺、骨骼肌匀浆或粪便悬浮液的猪未能产生抗HEV抗体。这些发现表明，在感染早期（3-20 DPI），猪 HEV 存在通过受感染猪的肝组织传播的潜在风险，并且体外 RT-PCR 测定与猪生物测定具有良好的相关性。 (C) 2002 Elsevier Science B.V. 保留所有权利。
The objective of this study was to assess the risk of transmission of swine hepatitis E virus (swine HEV) to naive pigs by inoculation with tissues or feces collected from pigs infected experimentally with swine HEV. Seventy-five, 3-week-old pigs were assigned randomly to 24 groups of 3-4 pigs and inoculated with homogenates of tissues (liver, heart, pancreas, or skeletal muscle) or a suspension of feces from swine HEV-infected pigs collected at 3, 7, 14, 20, 27. or 55 days post inoculation (DPI). Each inoculum was prepared as a 10% suspension (w/v) in PBS buffer and tested by a semi-quantitative RT-PCR for swine HEV RNA and by the swine bioassay. The inoculation route was intravenous for liver, heart and pancreas, and via stomach tube for skeletal muscle and fecal suspension. The liver homogenate inocula and feces collected at 3-7 and 14-20 DPI were positive for swine HEV RNA by RT-PCR. The pigs inoculated with liver homogenates collected at 3-7 and 14-20 DPI developed anti-HEV antibodies and swine HEV RNA was detected in their sera. Pigs inoculated with heart, pancreas, skeletal muscle homogenates or fecal suspensions failed to develop anti-HEV antibodies. These findings suggest that there is a potential risk of transmission of swine HEV via liver tissue from infected pigs in the early stages (3-20 DPI) of infection and the in vitro RT-PCR assay correlates well with the swine bioassay. (C) 2002 Elsevier Science B.V. All rights reserved.