Puerarin ameliorates skeletal muscle wasting and fiber type transformation in STZ-induced type 1 diabetic rats.
Puerarin ameliorates skeletal muscle wasting and fiber type transformation in STZ-induced type 1 diabetic rats.
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DOI：
10.1016/j.biopha.2020.110977
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发表时间：
2020-11
期刊：
影响因子：
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通讯作者：
Lin Yin;Xi Chen;Na Li;Wei-hua Jia;Nuo-qi Wang;Bi-yu Hou;Haiguang Yang;Li Zhang;G. Qiang-G.-Qi
中科院分区：
文献类型：
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作者：
Lin Yin;Xi Chen;Na Li;Wei-hua Jia;Nuo-qi Wang;Bi-yu Hou;Haiguang Yang;Li Zhang;G. Qiang-G.-Qi
Puerarin is an isoflavonoid extracted fromPueraria lobatewith extensive pharmacological effects in traditional Chinese medicine. The evidence implicates that puerarin mitigates hyperglycemia and various relevant complications. Here, the effect of puerarin on skeletal muscle wasting induced by type 1 diabetes (T1D) was explored. Streptozotocin (STZ)-induced T1D male Sprague Dawley (SD) rats were used in this study. Muscle strength, weight and size were measured. L6 rat skeletal muscle cells were applied forin vitrostudy. Our results showed that eight-week oral puerarin administration (100 mg/kg) increased muscle strengths and weights accompanied by enhanced skeletal muscle cross-sectional areas in diabetic rats. Simultaneously, puerarin also reduced expressions of several muscle wasting marker genes including F-box only protein 32 (Atrogin-1) and muscle-specific RING-finger 1 (Murf-1) in diabetic group bothin vitroandin vivo.Transformation from type I fibers (slow muscle) to type II fibers (fast muscle) were also observed under puerarin administration in diabetic rats. Puerarin promoted Akt/mTOR while inhibited LC3/p62 signaling pathway in skeletal muscle cells. In conclusion, our study showed that puerarin mitigated skeletal muscle wasting in T1D rats and closely related with Akt/mTOR activation and autophagy inhibition. Whether this effect in murine applies to humans remains to be determined.
