Fabricating electrospun cellulose nanofibre adsorbents for ion-exchange chromatography.
Fabricating electrospun cellulose nanofibre adsorbents for ion-exchange chromatography.
复制标题
DOI：
10.1016/j.chroma.2014.12.010
复制
发表时间：
2015-01-09
期刊：
影响因子：
--
通讯作者：
Bracewell DG
中科院分区：
文献类型：
--
作者：
Dods SR;Hardick O;Stevens B;Bracewell DG
DEAE 和 COO 官能化的优化化学方案。静电纺丝后施加的压缩提高了机械强度。增加压缩力和床层会降低结合能力。蛋白质分离是生物制药制造中不可或缺的步骤，而扩散限制填充床色谱仍然是行业的默认选择。使用对流传质介质的快速结合-洗脱分离通过在高流速下操作提供了生产力优势。电纺纳米纤维吸附剂是一种具有高表面积和孔隙率的非织造纤维基质，此前曾作为生物分离介质进行过研究。使用二乙氨基乙基（DEAE）或羧酸酯（COO）功能化研究了静电纺丝醋酸纤维素纳米纤维后压缩层和床层以及后续热处理的影响。通过压缩负载测量和比较移动床压力，在压缩 1、5 和 10 MPa 时，COO 吸附剂分别比 DEAE 高 30%、70% 和 90%，这归因于亲水 COO 基团的溶胀效应。在正常结合条件下，在 2000 至 12,000 CV/h（2 s 和 0.3 s 停留时间）之间测量 10% 突破时的动态结合容量 (DBC)，DBC 随着反应物浓度的增加而增加，DEAE 为 4 至 12 mg BSA/mL，COO 吸附剂为 10 至 21 mg 溶菌酶/mL。比较静电纺丝后施加的压缩负载的能力表明，测试的最低负载 1 MPa，DEAE 和 COO 吸附剂产生的 DBC 最高，分别为 20 mg BSA/mL 和 27 mg 溶菌酶/mL。在 1 MPa 时，测试的最低流速下的 DBC 最高，但流速高于 2000 CV/h 时则稳定。对于 5 MPa 和 10 MPa 的压缩负载，由于纳米纤维填充和表面积减少，吸附剂记录的 DBC 低于 1 MPa。将床层数从 4 层增加到 12 层表明两种吸附剂的 DBC 均减少。记录拉伸强度以指示吸附剂的机械稳健性，并且与将纳米纤维吸附剂包装在大规模配置（例如褶皱盒）中相关。与未压缩的吸附剂相比，两种功能化在 1、5 和 10 MPa 的压缩下分别增加了 30%、110% 和 110%。所提供的数据表明，容量和机械强度可以通过静电纺丝后的压缩来平衡，并且特定于不同的功能化。这种权衡对于将纳米纤维吸附剂开发成不同的填充结构以在生物分离中应用和扩大规模至关重要。
Optimised chemistry protocols of DEAE and COO functionalisations. Compression applied after electrospinning improved mechanical strength. Increasing compression and bed layers lowered binding capacities. Protein separation is an integral step in biopharmaceutical manufacture with diffusion-limited packed bed chromatography remaining the default choice for industry. Rapid bind-elute separation using convective mass transfer media offers advantages in productivity by operating at high flowrates. Electrospun nanofibre adsorbents are a non-woven fibre matrix of high surface area and porosity previously investigated as a bioseparation medium. The effects of compression and bed layers, and subsequent heat treatment after electrospinning cellulose acetate nanofibres were investigated using diethylaminoethyl (DEAE) or carboxylate (COO) functionalisations. Transbed pressures were measured and compared by compression load, COO adsorbents were 30%, 70% and 90% higher than DEAE for compressions 1, 5 and 10 MPa, respectively, which was attributed to the swelling effect of hydrophilic COO groups. Dynamic binding capacities (DBCs) at 10% breakthrough were measured between 2000 and 12,000 CV/h (2 s and 0.3 s residence times) under normal binding conditions, and DBCs increased with reactant concentration from 4 to 12 mg BSA/mL for DEAE and from 10 to 21 mg lysozyme/mL for COO adsorbents. Comparing capacities of compression loads applied after electrospinning showed that the lowest load tested, 1 MPa, yielded the highest DBCs for DEAE and COO adsorbents at 20 mg BSA/mL and 27 mg lysozyme/mL, respectively. At 1 MPa, DBCs were the highest for the lowest flowrate tested but stabilised for flowrates above 2000 CV/h. For compression loads of 5 MPa and 10 MPa, adsorbents recorded lower DBCs than 1 MPa as a result of nanofibre packing and reduced surface area. Increasing the number of bed layers from 4 to 12 showed decreasing DBCs for both adsorbents. Tensile strengths were recorded to indicate the mechanical robustness of the adsorbent and be related to packing the nanofibre adsorbents in large scale configurations such as pleated cartridges. Compared with an uncompressed adsorbent, compressions of 1, 5 and 10 MPa showed increases of 30%, 110% and 110%, respectively, for both functionalisations. The data presented show that capacity and mechanical strength can be balanced through compression after electrospinning and is particular to different functionalisations. This trade-off is critical to the development of nanofibre adsorbents into different packing configurations for application and scale up in bioseparation.
登录
查看更多内容
影响因子：
3.8
作者：
Bhut, Bharat V.;Weaver, Justin;Husson, Scott M.
通讯作者：
Husson, Scott M.
影响因子：
3.8
作者：
CHEN, LF;TSAO, GT
通讯作者：
TSAO, GT
影响因子：
5.7
作者：
Hirota, Masayuki;Tamura, Naoyuki;Isogai, Akira
通讯作者：
Isogai, Akira
影响因子：
4.1
作者：
Staby, Arne;Jacobsen, Jan H.;Jensen, Inge Holm
通讯作者：
Jensen, Inge Holm
影响因子：
1.8
作者：
Ward, William W.;Swiatek, Gavin
通讯作者：
Swiatek, Gavin