Surfactant-induced bacterial community changes correlated with increased polycyclic aromatic hydrocarbon degradation in contaminated soil.
Surfactant-induced bacterial community changes correlated with increased polycyclic aromatic hydrocarbon degradation in contaminated soil.
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DOI：
10.1007/s00253-016-7867-z
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发表时间：
2016-12
影响因子：
5
通讯作者：
Aitken MD
中科院分区：
文献类型：
--
作者：
Singleton DR;Adrion AC;Aitken MD
生物修复作为一种从污染环境中去除多环芳烃（PAHs）的方法，因其对潜在致癌但生物可利用性较低的高分子量（HMW）化合物的去除效果较差而受到批评。作为对这一限制的部分补救措施，我们研究了表面活性剂添加在亚胶束浓度污染土壤，以提高传统的好氧生物修复后剩余的多环芳烃的生物降解。我们证明了使用两种非离子表面活性剂，聚氧乙烯（4）月桂基醚（Brij 30）和聚氧乙烯山梨醇六油酸酯（POESH），4-和5-环多环芳烃的去除率增加，并分析了与这些条件相关的细菌群落变化。八组丰富的细菌被认为可能参与增加HMW PAH的去除。一组未分类的Alphaproteobacteria和Phenylobacterium属的成员，特别是表现出显着增加的相对丰度在两个条件下，表现出增加的PAH去除。其他涉及的群体包括Sedinibacterium，Terrimonas，Acidovorax和Luteimonas属的成员，以及Chitinophagaceae和Bradyrhizobiaceae家族中的未表征生物。有针对性的隔离确定了一个子集的社会可能使用的表面活性剂作为生长基质，但很少有菌株表现出PAH降解能力。从Acidovorax和未表征的Bradyrhizobiaceae组回收的分离物表明，这些组的丰度可能是由于表面活性剂的生长。了解特定的细菌负责高分子量多环芳烃去除在自然和工程系统和他们的刺激，如表面活性剂修正案的反应，可以提高生物修复的效果在处理污染的环境介质。
Bioremediation as a method for removing polycyclic aromatic hydrocarbons (PAHs) from contaminated environments has been criticized for poor removal of potentially carcinogenic but less bioavailable high-molecular-weight (HMW) compounds. As a partial remedy to this constraint, we studied surfactant addition at sub-micellar concentrations to contaminated soil to enhance the biodegradation of PAHs remaining after conventional aerobic bioremediation. We demonstrated increased removal of 4- and 5-ring PAHs using two nonionic surfactants, polyoxyethylene(4)lauryl ether (Brij 30) and polyoxyethylene sorbitol hexaoleate (POESH), and analyzed bacterial community shifts associated with those conditions. Eight groups of abundant bacteria were implicated as potentially being involved in increased HMW PAH removal. A group of unclassified Alphaproteobacteria and members of the Phenylobacterium genus in particular showed significantly increased relative abundance in the two conditions exhibiting increased PAH removal. Other implicated groups included members of the Sediminibacterium, Terrimonas, Acidovorax, and Luteimonas genera, as well as uncharacterized organisms within the families Chitinophagaceae and Bradyrhizobiaceae. Targeted isolation identified a subset of the community likely using the surfactants as a growth substrate but few of the isolates exhibited PAH-degradation capability. Isolates recovered from the Acidovorax and uncharacterized Bradyrhizobiaceae groups suggest the abundance of those groups may have been attributable to growth on surfactants. Understanding the specific bacteria responsible for HMW PAH removal in natural and engineered systems and their response to stimuli such as surfactant amendment may improve bioremediation efficacy during treatment of contaminated environmental media.