AN IMMUNOCYTOCHEMICAL STUDY ON THE OCCURRENCE OF LIVER FATTY-ACID-BINDING PROTEIN IN THE DIGESTIVE ORGANS OF RATS - SPECIFIC LOCALIZATION IN THE D-CELLS AND BRUSH CELLS
AN IMMUNOCYTOCHEMICAL STUDY ON THE OCCURRENCE OF LIVER FATTY-ACID-BINDING PROTEIN IN THE DIGESTIVE ORGANS OF RATS - SPECIFIC LOCALIZATION IN THE D-CELLS AND BRUSH CELLS
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DOI：
10.1159/000146915
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发表时间：
1990-05-01
期刊：
影响因子：
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通讯作者：
KONDO, H
中科院分区：
文献类型：
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作者：
ISEKI, S;KONDO, H
大鼠肝脂肪酸结合蛋白（L-FABP）最初是从肝实质细胞中分离出来的，后来也在肠吸收细胞中发现。通过光镜和电镜免疫细胞化学方法，我们研究了L-FABP在大鼠整个消化系统中的分布，并揭示了另外两种细胞类型，即内分泌D细胞和刷状细胞，对L-FABP具有特异性免疫反应性。免疫反应性D细胞，确定了同时免疫反应生长抑素和特征性内分泌颗粒，被发现在胃上皮和胰岛。免疫反应刷状细胞主要分布于胃上皮，也见于直肠和胆总管上皮。几乎所有的免疫反应刷状细胞有一个薄的突起与基底膜接触。刷状细胞内未见类似内分泌细胞的致密核心分泌颗粒。目前的研究结果表明，L-FABP是一个有用的胃肠D细胞和刷状细胞，特别是后者的标志物，证实刷状细胞是一个独特的实体不同于任何其他类型的细胞在胃肠上皮。此外，FABP可能参与这些细胞类型与脂肪酸代谢相关的特定功能。
Rat liver fatty-acid-binding protein (L-FABP) was originally isolated from the liver parenchymal cells and later found also in the intestinal absorptive cells. By light-and electron-microscopic immunocytochemistry we examined the distribution of L-FABP in the entire digestive system of the rat and revealed two other cell types, i.e. the endocrine D cell and the brush cell, to be specifically immunoreactive for L-FABP. The immunoreactive D cells, identified by the simultaneous immunoreactivity for somatostatin and by characteristic endocrine granules, were found in the stomach epithelium and pancreatic islets. The immunoreactive brush cells, identified by the ultrastructural features of cell apex, were found primarily in the stomach epithelium and also in the epithelia of the rectum and common bile duct. Almost all immunoreactive brush cells had a thin process in contact with the basement membrane. No secretory granules with dense cores similar to those of the endocrine cells were observed in the brush cells. The present findings reveal L-FABP to be a useful marker of the gastrointestinal D cells and brush cells, especially of the latter, confirming that the brush cell is a distinct entity different from any other cell types in the gastrointesinal epithelia. Furthermore, FABP may be involved in the specific functions of these cell types related to fatty acid metabolism.