Biological formation of ethylene.
Biological formation of ethylene.
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DOI：
10.1039/d3cb00066d
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发表时间：
2023-08-30
影响因子：
4.1
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中科院分区：
文献类型：
--
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本文综述了乙烯的两个主要生物来源--乙烯形成酶(EFE)和1-氨基环丙烷-1-羧酸(ACC)氧化酶(ACCO)的结构、生化特性和作用机制。在选定的细菌和真菌中发现了EFe，它催化两个反应：(1)氧依赖的2-氧戊二酸(2OG)转化为乙烯和三个分子的二氧化碳/重碳酸盐；(2)2OG的氧化脱羧基，同时将L-精氨酸转化为胍和L-Δ1-吡咯烷-5-羧酸。Acco存在于植物中，它通过将ACC、O2和一种外部还原剂转化为乙烯、HCN、CO2和水来制造植物激素。尽管EFE和ACCO催化不同的化学反应，但它们在序列和结构上是相关的，而且两种酶都需要Fe(II)才能发挥活性。我们对EFE的理解从实验和计算两个方面取得了进展，澄清了这种酶是如何催化其双重反应的。在已发表的ACCO反应机理研究的基础上，并注意到该酶与EFE之间的相似性，我们提出了一种新的ACCO反应机理。由乙烯形成酶(EFE)和1-氨基环丙烷-1-羧酸氧化酶(ACCO)形成的乙烯。
This review summarizes the structures, biochemical properties, and mechanisms of two major biological sources of ethylene, the ethylene-forming enzyme (EFE) and 1-aminocyclopropane-1-carboxylic acid (ACC) oxidase (ACCO). EFE is found in selected bacteria and fungi where it catalyzes two reactions: (1) the oxygen-dependent conversion of 2-oxoglutarate (2OG) to ethylene plus three molecules of CO2/bicarbonate and (2) the oxidative decarboxylation of 2OG while transforming l-arginine to guanidine and l-Δ1-pyrroline-5-carboxylic acid. ACCO is present in plants where it makes the plant hormone by transforming ACC, O2, and an external reductant to ethylene, HCN, CO2, and water. Despite catalyzing distinct chemical reactions, EFE and ACCO are related in sequence and structure, and both enzymes require Fe(ii) for their activity. Advances in our understanding of EFE, derived from both experimental and computational approaches, have clarified how this enzyme catalyzes its dual reactions. Drawing on the published mechanistic studies of ACCO and noting the parallels between this enzyme and EFE, we propose a novel reaction mechanism for ACCO. Ethylene formation by the ethylene-forming enzyme (EFE) and 1-aminocyclopropane-1-carboxylate oxidase (ACCO).