A bromodeoxyuridine (BUdR)-mithramycin technique for detecting cycling and non-cycling cells by flow microfluorometry.
A bromodeoxyuridine (BUdR)-mithramycin technique for detecting cycling and non-cycling cells by flow microfluorometry.
复制标题
一种溴脱氧尿苷 (BUdR)-神霉素技术，用于通过流式显微荧光法检测循环和非循环细胞。
DOI：
10.1016/0014-4827(77)90024-6
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发表时间：
1977
影响因子：
3.7
通讯作者：
D. Swartzendruber
中科院分区：
文献类型：
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作者：
D. Swartzendruber
溴脱氧尿苷 (BUdR) 掺入细胞 DNA 会导致光神霉素染色细胞的荧光增强。在 30 μM BUdR 存在的情况下，流式显微荧光测定法可用于区分未复制的细胞、已复制一次的细胞以及已复制两次或多次的细胞。与现有方案相比，该技术具有两个优点：（1）光神霉素染色剂提供阳性荧光信号，而不是像赫斯特染色剂那样提供阴性信号； (2) 光神霉素染色后，可以在不到10分钟的时间内分析细胞周期任何阶段的数十万个细胞。本文描述的 BUdR-光神霉素技术是研究含有分裂和非分裂细胞的异质群体的细胞周期动力学的有用工具。
Bromodeoxyuridine (BUdR) incorporation into cellular DNA results in an increase in fluorescence of mithramycin-stained cells. Flow microfluorometry can be used to distinguish among cells which have not replicated, cells which have replicated once, and cells which have replicated two or more times in the presence of 30 μM BUdR. This technique offers two advantages over present protocols: (1) a positive fluorescent signal is provided by the mithramycin stain rather than a negative signal, as with the Hoechst stain; (2) after mithramycin staining, hundreds of thousands of cells in any phase of the cell cycle can be analyzed in less than 10 min. The BUdR-mithramycin technique described herein is a useful tool for studying the cell-cycle kinetics of heterogeneous populations containing dividing and non-dividing cells.