Developmental properties of the Hertwig's epithelial root sheath in mice
Developmental properties of the Hertwig's epithelial root sheath in mice
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DOI：
10.1177/154405910408300906
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发表时间：
2004-09-01
影响因子：
7.6
通讯作者：
Jung, HS
中科院分区：
文献类型：
--
作者：
Yamamoto, H;Cho, SW;Jung, HS
Hertwig's epithelial root sheath（HERS）在牙根形成中起重要作用。在这项研究中，我们研究了小鼠第一磨牙的牙根形成，重点是细胞增殖，细胞死亡，细胞迁移和信号分子的表达模式，包括PN 8和PN 26之间的糖蛋白和蛋白聚糖。HERS细胞的数量减少根形成过程中，虽然HERS保留总长度，直到PN 15。在根形成过程中没有发生HERS细胞的迁移。此外，层粘连蛋白β-3和syndecan- 1在HERS中的免疫阳性反应表明细胞粘附和细胞增殖对于HERS的发展是必不可少的。BMP- 2、BMP- 4和Msx- 2在根形成过程中在HERS细胞中表达。我们还开发了一种体外培养系统，用于调查牙周组织，并建议该系统提供了一个很好的车辆进行充分的探索，从而提高理解，牙周组织的发展和再生。总之，我们的研究结果提供了一个全面的模型描述脊椎动物早期根发育的形态发生。
Hertwig's epithelial root sheath ( HERS) plays an important role in tooth root formation. In this study, we examined root formation of the first molar in mice, focusing on cell proliferation, cell death, cell migration, and the expression patterns of the signaling molecules, including glycoproteins and proteoglycans between PN8 and PN26. The number of HERS cells decreased during root formation, although HERS retained total length until PN15. The migration of HERS cells did not occur during root formation. Moreover, the immunopositive reaction of laminin beta- 3 and syndecan- 1 in HERS indicates that both cell adhesion and cell proliferation are essential for HERS development. Bmp- 2, Bmp- 4, and Msx- 2 were expressed in HERS cells during root formation. We also developed an in vitro culture system for investigating the periodontium and suggest that this system provides an excellent vehicle for full exploration, and hence improved understanding, of the development and regeneration of the periodontium. Together, our results provide a comprehensive model describing the morphogenesis of early root development in vertebrates.