ANTIOXIDANT ENZYME LEVELS AS A FUNCTION OF GROWTH-STATE IN CELL-CULTURE
ANTIOXIDANT ENZYME LEVELS AS A FUNCTION OF GROWTH-STATE IN CELL-CULTURE
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DOI：
10.1016/0891-5849(95)00012-m
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发表时间：
1995-07-01
影响因子：
7.4
通讯作者：
OBERLEY, LW
中科院分区：
文献类型：
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作者：
OBERLEY, TD;SCHULTZ, JL;OBERLEY, LW
监测锰超氧化物歧化酶（MnSOD）水平随培养时间的变化，以确定这些水平在对数生长时与细胞表现出生长密度限制时是否发生变化。为了进行比较，还评估了抗氧化酶铜、锌超氧化物歧化酶 (CuZnSOD)、过氧化氢酶和谷胱甘肽过氧化物酶的活性。研究了四种细胞系，其中两种表现出生长密度限制，另两种则没有。每个细胞系都显示出独特的抗氧化酶谱。表现出生长密度限制的两种细胞系在细胞在培养物中停止增殖时也表现出MnSOD的诱导，而其他两种细胞系没有表现出MnSOD的诱导。生长密度限制与其他抗氧化酶的活性之间没有严格的相关性。为了确定 SOD 是否随细胞周期的不同阶段而变化，使用血清饥饿对 NIH/3T3 细胞进行同步化，然后在静止期 (G(0)) 和 DNA 合成期 (S 期) 测量 SOD 活性。与 G(0) 相比，S 期 MnSOD 减少，而与 G(0) 相比，S 期 CuZnSOD 增加，表明 SOD 活性随细胞周期不同阶段的变化而变化。这项研究表明，MnSOD 增加可能与细胞增殖减少相关，并表明细胞周期中 SOD 活性发生显着变化。
Manganese superoxide dismutase (MnSOD) levels were monitored as a function of time in culture to determine whether these levels were altered at logarithmic growth versus when the cells exhibited density limitation of growth. For comparison, activities of the antioxidant enzymes copper, zinc superoxide dismutase (CuZnSOD), catalase, and glutathione peroxidase were also evaluated. Four cell lines were studied, two of which exhibited density limitation of growth and two of which did not. Each cell line showed a unique antioxidant enzyme profile. The two cell lines that showed density limitation of growth also demonstrated induction of MnSOD at the time when the cells stopped proliferating in culture, whereas the other two cell lines did not show induction of MnSOD. There was no strict correlation between density limitation of growth and activities of the other antioxidant enzymes. To determine whether SOD varied with various phases of the cell cycle, NIH/3T3 cells were synchronized using serum starvation, and then SOD activities were measured during quiescence (G(0)) and the phase of DNA synthesis (S-phase). MnSOD was decreased during S-phase compared with G(0), whereas CuZnSOD was increased during S-phase compared with G(0), demonstrating alteration of SOD activities with varying phases of the cell cycle. This study suggests the possibility that increased MnSOD may correlate with decreased cell proliferation and suggests significant alterations in SOD activities during the cell cycle.