Fluorescent Core-Shell Star Polymers Based Bioassays for Ultrasensitive DNA Detection by Surface Plasmon Fluorescence Spectroscopy
Fluorescent Core-Shell Star Polymers Based Bioassays for Ultrasensitive DNA Detection by Surface Plasmon Fluorescence Spectroscopy
复制标题
基于荧光核壳星形聚合物的表面等离子体荧光光谱超灵敏 DNA 检测生物测定
DOI：
10.1002/marc.201000788
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发表时间：
2011-04-19
影响因子：
4.6
通讯作者：
Muellen, Klaus
中科院分区：
文献类型：
--
作者：
Feng, Chuan Liang;Yin, Meizhen;Muellen, Klaus
用逐层(LBL)方法在银衬底上制备了与磷树状分子层相邻的星形聚合物(FSP)施主的多层膜。利用表面等离子体增强荧光光谱(SPFS)技术，在543 nm激发下，实现了基于能量转移(ET)增强光场的时间分辨超灵敏和选择性的DNA靶标检测。检测限比632 nm激发下提高了约8个数量级，这归因于在632 nm激发下没有从施主到受主的能量转移，从而导致了较弱的检测信号。
Multilayers containing a perylene diimide labelled star polymers (FSP) donor adjacent to phosphorus dendrimer layer on a silver substrate were constructed by layer by layer (LBL) approach. Using Surface Plasmon Enhanced Fluorescence Spectroscopy (SPFS) technique, a time-resolved ultrasensitive and selective detection of DNA targets relying on enhanced optical fields associated with energy transfer (ET) were achieved under the excitation at 543 nm. The detection limit is about 8 orders of magnitude better than the achieved one under the excitation at 632 nm, which is ascribed to no energy transfer from the donor to the acceptor under the excitation at 632 nm, resulting in much weak detection signal in turn.