Exploitation of a novel polysaccharide nanogel cross‐linking membrane for guided bone regeneration (GBR)
Exploitation of a novel polysaccharide nanogel cross‐linking membrane for guided bone regeneration (GBR)
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DOI：
10.1002/term.475
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发表时间：
2012-08
影响因子：
3.3
通讯作者：
T. Miyahara;Myat Nyan;A. Shimoda;Yuka Yamamoto;S. Kuroda;M. Shiota;K. Akiyoshi;S. Kasugai
中科院分区：
文献类型：
--
作者：
T. Miyahara;Myat Nyan;A. Shimoda;Yuka Yamamoto;S. Kuroda;M. Shiota;K. Akiyoshi;S. Kasugai
胆固醇普鲁兰多糖（CHP）纳米凝胶是一种合成的可降解生物材料，具有良好的生物相容性。引导骨再生（GBR）是一种骨增量技术，其中使用膜来创建和保持隔离的再生空间。本研究的目的是评估新型CHP纳米凝胶膜在GBR中的效果。使用36只成年Wistar大鼠，用骨环钻创建直径为5 mm的双侧对称全层顶骨缺损。每个缺损用胶原蛋白膜或CHP纳米凝胶膜覆盖或不使用任何膜而未处理。在第2、4和8周处死动物，并进行放射学和组织学分析。此外，在将人血清与CHP纳米凝胶或胶原一起孵育后，使用ELISA测量血清中PDGF的量。在第2周和第4周，纳米凝胶组的新骨形成（就骨体积而言）高于对照组或胶原组。第8周时，两个膜组的骨体积均高于对照组。值得注意的是，纳米凝胶组中骨缺损中新形成的骨是均匀的，在组织学上与原始骨无法区分，而在胶原蛋白组中，新骨显示出与原始骨完全不同的不规则结构。与CHP纳米凝胶孵育后，血清中PDGF的量显著降低。CHP纳米凝胶GBR膜有利地刺激骨再生，其中可能涉及CHP纳米凝胶的独特特性，即内源性生长因子的储存。版权所有© 2011约翰威利父子有限公司.
Cholesterol‐bearing pullulan (CHP) nanogel is a synthetic degradable biomaterial for drug delivery with high biocompatibility. Guided bone regeneration (GBR) is a bone augmentation technique in which a membrane is used to create and keep a secluded regenerative space. The purpose of the present study was to evaluate the effects of the novel CHP nanogel membrane in GBR. Thirty‐six adult Wistar rats were used and bilaterally symmetrical full‐thickness parietal bone defects of 5 mm diameter were created with a bone trephine burr. Each defect was covered with the collagen membrane or the CHP nanogel membrane or untreated without any membrane. The animals were sacrificed at 2, 4 and 8 weeks and analysed radiologically and histologically. Furthermore, after incubating human serum with CHP nanogel or collagen, the amount of PDGF in the serum was measured using ELISA. New bone formation in terms of bone volume was higher in the nanogel group than in the control or collagen groups at 2 and 4 weeks. At 8 weeks, both membrane groups showed higher bone volumes than the control group. Notably, the newly‐formed bone in the bone defect in the nanogel group was uniform and histologically indistinguishable from the original bone, whereas in the collagen group the new bone showed an irregular structure that was completely different from the original bone. After incubating with CHP nanogel, the amount of PDGF in the serum decreased significantly. CHP nanogel GBR membrane favourably stimulated bone regeneration, in which a unique characteristic of CHP nanogel, the storage of endogenous growth factors, was likely implicated. Copyright © 2011 John Wiley & Sons, Ltd.