An in vitro assay procedure to test chemotherapeutic drugs on cells from human solid tumors.
An in vitro assay procedure to test chemotherapeutic drugs on cells from human solid tumors.
复制标题
用于测试来自人类实体瘤细胞的化疗药物的体外测定程序。
作者：
S. Shrivastav;R. A. Bonar;K. R. Stone;D. Paulson
建立了一种测定人肿瘤细胞化疗药物敏感性的体外实验方法。这项检测利用了新鲜从肿瘤组织中分离出来的活细胞，这些细胞在体外培养了很短的时间。检测药物对放射性标记前体掺入DNA、RNA和蛋白质的抑制作用。该分析方法对治疗范围内的化疗药物浓度敏感，当用相同肿瘤细胞群的复制品进行测试时，该方法是可重复性的。
An in vitro assay was developed to measure the chemotherapeutic drug susceptibility of cells from human tumors. The assay utilized live cells, freshly isolated from tumor tissue, which were incubated for a short period in vitro. The drug-induced inhibition of incorporation of radiolabeled precursor into DNA, RNA, and protein was measured. The assay is sensitive to concentrations of chemotherapeutic drugs in the therapeutic range and is reproducible when tested with replicates of the same tumor cell population.