Serologic and hexon phylogenetic analysis of ruminant adenoviruses
Serologic and hexon phylogenetic analysis of ruminant adenoviruses
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DOI：
10.1007/s00705-008-0063-4
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发表时间：
2008-05-01
影响因子：
2.7
通讯作者：
Hobbs, L. A.
中科院分区：
文献类型：
--
作者：
Lehmkuhl, Howard D.;Hobbs, L. A.
本研究的目的是根据推导的Hexon基因氨基酸序列，确定反刍动物腺病毒之间的抗原关系，并确定它们之间的系统发育关系。交叉中和试验结果显示牛腺病毒6型(BAdV-6)、BAdV-7、羊腺病毒7型(OAdV-7)、山羊腺病毒1型(GAdV-1)和鹿腺病毒(OdAdV-1)之间存在单向或双向的抗原性关系。BAdV-1、BAdV-5、BAdV-8与其他反肠腺病毒之间未观察到抗原交叉反应。两套用于比较研究的聚合酶链式反应引物，一套用于肥大病毒，另一套用于腺病毒，它们扩增了大约2700个碱基的六邻体基因区域。推导的六邻体氨基酸序列的系统发育分析将反刍动物的腺病毒聚在腺病毒科的乳头腺病毒和腺病毒属分支上。通过对BAdV-6、BAdV-7和BAdV-7推导的六邻体氨基酸序列的系统发育距离矩阵分析，支持了BAdV-6和BAdV-7作为腺病毒属成员的最新分类。此外，我们建议BAdV-6和BAdV-7分别被确认为牛腺病毒新种--牛腺病毒E和牛腺病毒F。OdAdV-1与新种Odocoileus腺病毒A属同源，OAdV-6和GAdV-2分别属于新种绵羊腺病毒C和山羊腺病毒A。
The objectives of this study were to determine the antigenic relationship among ruminant adenoviruses and determine their phylogenetic relationship based on the deduced hexon gene amino acid sequence. Results of reciprocal cross-neutralization tests demonstrated antigenic relationships in either one or both directions among bovine adenovirus type 6 (BAdV-6), BAdV-7, ovine adenovirus type 7 (OAdV-7), caprine adenovirus type 1 (GAdV-1), and deer adenovirus (Odocoileus adenovirus 1, OdAdV-1). No antigenic cross-reactivity was observed among BAdV-1 through -5, and -8 and the other putative ruminant adenoviruses. Two PCR primer sets, one for mastadenovirus and atadenovirus that amplified an approximately 2,700-bp region in the hexon genes were used for comparative studies. Phylogenetic analysis of the deduced hexon amino acid sequences clustered the ruminant adenoviruses on the Mastadenovirus and Atadenovirus genus branches of the Adenoviridae tree. The recent classification of BAdV-6, and -7 as members of the genus Atadenovirus was supported by phylogenetic distance matrix analysis of their deduced hexon amino acid sequences. Further, we propose that BAdV-6 and -7 be recognized as members of new Atadenovirus species, Bovine adenovirus E and Bovine adenovirus F, respectively. Phylogenetic analysis of OdAdV-1 places this virus in the genus Atadenovirus with a proposed new species Odocoileus adenovirus A. OAdV-6 and GAdV-2 are proposed as members of new Mastadenovirus species Ovine adenovirus C and Goat adenovirus A, respectively.